TPH2基因单核苷酸多态性与双相情感障碍及其自杀行为的关系  

Corelation Among Single Nucleotide Polymorphism of Tryptophan Hydroxylase Isoform (TPH2) Gene rs7305115,Bipolar Disorder and Suicidal Behavior

作　　者：张玉琦[1] 袁国桢[1] 李桂林[1] 姚建军[1] 程灶火[1] 储兴[1] 季庆[1] 刘朝军 刘庆海 王爱荣 史桂芝[3] 王宝恒 成义仁 李克[3] 

机构地区：[1]中国.南京医科大学附属无锡精神卫生中心,江苏无锡214151 [2]山东省安康院 [3]汕头大学医学院分子遗传学教研室

出　　处：《中国健康心理学杂志》2009年第7期771-774,共4页China Journal of Health Psychology

基　　金：江苏省无锡市社会发展科技指导性计划项目(CLZ00721);广东省汕头市科技计划项目(汕府科[2004]102号)

摘　　要：目的探讨色氨酸羟化酶2(TPH2)基因rs7305115单核苷酸多态性与双相情感障碍及自杀行为的关系。方法提取205例双相情感障碍患者和225名健康对照者基因组DNA,采用聚合酶链反应(polymerase chain reaction PCR)扩增包括TPH2基因rs7305115位点的312bp基因组DNA片段,PCR产物直接测序。结果在第7外显子周围未发现其它的单核苷酸多态性。双相情感障碍患者和健康对照者TPH2rs7305115基因型和等位基因频率无统计学意义的差别(P>0.05),但患者组内有自杀行为的个体携带基因型AA的频率及等位基因A的频率均较低,两组比较差异有统计学意义(P<0.05)。结论TPH2基因rs7305115单核苷酸多态性与双相情感障碍无明显关联,与自杀行为有关联,其可能与双相情感障碍自杀行为易感性相关。Objective To explore the relation among single nucleotide polymorphism of a novel tryptophan hydroxylase isoform （TPH2） gene rs7305115,bipolar disorder and suicidal behavior. Methods Specimens of peripheral blood were collected from 205 bipolar disorder and 225 controls. A novel tryptophan hydroxylase isoform （TPH2） gene rs7305115 in length 312bp was amplitied by Polvmerase chain reaction （PCR）, and the product was analyzed by direct sequencing. Results We did not discover new single nucleotide polymorphism. Compared with Control Group,no significant difference of genotypes and alleles of TPH2 gene rs7305115 single nucleotide polymorphism had been found in patient group（P〉0. 05）. However,there existed significant differences between suicide behavior and non suicide behavior in bipolar disorder patient in genotypes of TPH2 gene rs7305115A/A. Suicide behavior of bipolar disorder patients in AA genotypes was much lower than non suicide behavior of bipolar disorder patients （P〈0. 05）. Con- clusion TPH2 gene rs7305115 single nucleotide polymorphism may have no association with the susceptibility of bipolar disorder, but associated with suicide behavior in bipolar disorder. A allele may be one Of the risk factors for suicide behavior in bipolar disor- der.

关 键 词：双相情感障碍 自杀行为 TPH2基因 单核苷酸多态性 

分 类 号：R394[医药卫生—医学遗传学] R749.4[医药卫生—基础医学]