γ-环糊精-刚果红包合物与DNA的作用方式研究  

Research on the Interaction Mode Between Inclusion Complex γ-Cyclodextrin-Congo Red and Herring-sperm DNA

作　　者：费丹[1] 王兴明[1] 黎泓波[2] 胡亚敏[1] 于婷婷[1] 赵娜[1] 丁立生[3] 

机构地区：[1]西南科技大学材料科学与工程学院化学系,绵阳621010 [2]湖南大学化学化工学院,长沙410082 [3]中国科学院成都生物研究所,成都610041

出　　处：《武汉理工大学学报》2009年第16期58-62,共5页Journal of Wuhan University of Technology

基　　金：国家自然科学基金(30572254)

摘　　要：应用光谱法和电化学方法研究了γ-环糊精与刚果红的包合作用及包合物与鲱鱼精DNA的作用方式。结果表明,-γCD与刚果红形成1∶1包合物,包合常数K=1.22×103L/mol。包合物与DNA的结合比为n-γCD-CR∶nDNA=5∶1,25℃与37℃下结合常数分别为KΘ25℃=4.10×103L/mol,KΘ37℃=1.42×104L/mol。包合物与DNA间存在嵌插与静电作用,该过程为熵所驱动。Inclusion interaction of Congo red with γ-cyclodextrin and interaction mode between inclusion complex and herring-sperm DNA are researched by spectral and electrochemistry analysis. The result indicates that 1 ： 1 inclusion complexes between γ-CD and CR are formed, and the binding constant is K = 1.22 × 10^3 L/mol. The binding ratio of γ-CD-CR and herring-sperm DNA is nγ-CD-CR： n DNA = 5 ： 1, and the binding constant are Kθ25 ℃ = 4.10 ×10^3 L/rnol, Kθ 37℃ = 1.42 × 10^4 L/mol respectively. The main binding mode between γ-CD-CR and DNA is a mixed binding, including intercalation and electrostatic binding modes. Entropy is the primary driven power of this interaction.

关 键 词：γ环糊精 刚果红 包合物 鲱鱼精DNA 作用方式 

分 类 号：O629.74[理学—有机化学]