蛋白质飞行质谱技术在原发性肝癌诊断中的应用  

Application of surface enhanced laser desorption/ionization time-of-flight mass spectrometry technology in the diagnosis of primary liver carcinomas

作　　者：温树伟[1] 党之俊[1] 苑天文[1] 薛耀勤[1] 

机构地区：[1]山西省肿瘤医院介入科,太原030013

出　　处：《肿瘤研究与临床》2009年第8期463-465,共3页Cancer Research and Clinic

摘　　要：目的研究原发性肝癌患者与健康人血清蛋白质表达的差异性，寻找对原发性肝癌更有效的肿瘤诊断标志物。方法应用表面增强激光解析电离飞行时间质谱（SELDI—TOF—MS）技术检测50例原发性肝癌患者、50名健康对照者血清中的蛋白质谱，寻找有意义的蛋白质谱。同时采用双抗体夹心法检测血清甲胎蛋白（AFP）。结果原发性肝癌患者血清4个蛋白质峰与健康对照组比较差异有统计学意义。分别为：3354．71，8825．80（高表达），4345．08，13715．01（低表达）。从中筛选出质荷比（M／z）分别为3354．71、8825．80差别最显著的蛋白质峰，使用这两个蛋白质峰作为原发性肝癌的诊断模式，其灵敏度、特异度分别为90％（45／50），94％（47／50）。100例患者中，AFP阳性27例，灵敏度为54％（27／50），特异度为100％（30／30）。结论SELDI—TOF—MS蛋白质芯片技术在原发性肝癌的诊断及肿瘤特异性蛋白质生物标志的筛选中具有一定的价值，灵敏度和特异度较高，操作简单，正确诊断指数（r=83）优于AFP（r=53）。Objective To explore the tumor markers for the diagnosis of primary liver carcinomas （PLC） by detecting the serum protein spectrum differently expressed between PLC patients and healthy controls. Methods We detected the serum protein spectrum in 50 PLC patients and 50 healthy controls using surface enhanced laser desorption ionization time-of-flight mass spectrometry （SELDI-TOF-MS） technique and find the significant protein peaks. The serum Alpha-fetoprotein （AFP） levels in all 100 serum samples were also measured by ELISA. Results The protein peaks, which could discriminate healthy individuals from PLC patients, were detected. Four protein molecules （3354.71, 8825.80, 4345.08, 13 715.01） had a significant difference between PLC patients and the normal controls （P 〈 10-s）, indicating that these protein molecules might be a potential marker for PLC. The specificity and sensitivity of SELDI-TOF-MS were 94 % and 90 % respectively. Sixteen PLC patients were AFP positive and the sensitivity was 54 %（27/50）. Conclusion With a high specificity and sensitivity, the detection of serum protein spectrum can be performed easily and quickly by SELDI-TOF-MS technique, which provides a serological way in identifying PLC and most likely to benefit from AFP strategies.

关 键 词：肝肿瘤 蛋白质组学 光谱法 质量 基质辅助激光解析电离 

分 类 号：R735.7[医药卫生—肿瘤]