甘氨双唑钠联合顺铂对宫颈腺癌HeLa细胞敏感性的影响  

Effects of combination of sodium glycididazole with cisplatin on sensitivity of cervical carcinoma cell line HeLa

作　　者：叶红[1] 时艳梅[1] 肖长义[2] 刘秀娟[1] 李彩虹[1] 

机构地区：[1]三峡大学第一临床医学院,湖北省宜昌市443003 [2]三峡大学医学院,湖北省宜昌市443002

出　　处：《实用医学杂志》2009年第17期2911-2914,共4页The Journal of Practical Medicine

摘　　要：目的:甘氨双唑钠(CMNa)是中国研制的一类抗癌新药,在鼻咽癌、食管癌、肺癌等的放疗增敏研究中均已取得显著疗效,但对化疗药的增敏效果如何,目前仍不肯定。本研究以宫颈腺癌HeLa细胞为靶点,分析CMNa是否可影响宫颈腺癌细胞HeLa对化疗药物顺铂(DDP)的敏感性。方法:不同浓度DDP、CMNa及双药联合作用于对数生长期的HeLa细胞,通过形态学观察、四甲基偶氮唑盐法(MTT)细胞活力测定等检测对HeLa敏感性的影响。结果:不同浓度DDP对HeLa细胞生长均有抑制作用,而不同浓度CMNa抑制作用不明显,低浓度DDP与CMNa联合作用于细胞可增强DDP对HeLa细胞生长的抑制。结论:低浓度DDP与CMNa联合应用,不但提高了DDP对宫颈腺癌细胞的敏感性,而且降低了大剂量化疗药物的使用,减轻了患者的不良反应,可为宫颈癌化疗方案的制定提供理论指导。Objective Sodium glycididazole （CMNa） was a novel tumor radiosensitizer, used on radiosensitizing research of laryngeal carcinoma, pharyngeal cancer, and lung cancer. It had obtained remarkable effection. The effects of CMNa on chemotherapy was still not affirmed. This research took the cervical adenocarcinoma HeLa cells astarget cells, analyzed whether CMNa effected the chemosensitivity of cisplatin on cervical adenocarcinoma HeLa cells. Methods HeLa cells were subjected to cisplatin（DDP）, CMNa, and both of them in various concentrations for 24 hours, and the morphology of the HeLa cells was observed. MTr eolorimetry was used to determine the effects of the drugs on HeLa cells. Results The HeLa cells treated with different concentrations of cisptatin underwent cell proliferation inhibition. Various concentrations of CMNa were not effected the HeLa cells. CMNa could strengthen the effects of low-concentrated DDP on HeLa cells greatly. Conclusion The combination of CMNa and DDP could not only enhance the sensitivity of DDP to HeLa cells, but also decrease the dosage of DDP,which could provide theoretical guidance for cervical carcinoma.

关 键 词：宫颈肿瘤 顺铂 甘氨双唑钠 化疗增敏 

分 类 号：R737.33[医药卫生—肿瘤]