牛颌下腺粘蛋白中糖组成的毛细管气相色谱分析  

Composition Analysis of Carbohydrate Released from Bovine Submaxillary Mucin by Capillary Gas Chromatography

作　　者：徐桂芸[1] 常理文[1] 费丽华[2] 

机构地区：[1]中国科学院化学研究所,北京100080 [2]中国人民解放军总医院,北京100853

出　　处：《分析化学》1998年第8期922-926,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金;国家教委基金资助项目

摘　　要：根据各种糖苷键稳定性的差异，采用不同的酸解方法，使各种单糖从牛颌下腺粘蛋白上游离下来。纯化后衍生成三甲基硅醚或糖醇乙酸酯，应用毛细管气相色谱技术分析。结果表明，牛颌下腺粘蛋白中含三类糖，即唾液酸、中性糖和氨基糖。每种单糖的含量分别为５－Ｎ－乙酰神经氨酸４．３％；５－Ｎ一乙醇酸神经氨酸１９．０％；岩藻糖３．２％；半乳糖５．１％；Ｎ－乙酸氨基半乳糖１８．４％；Ｎ－乙酸氨基葡萄糖８．７％。The six monosaccharides including static acid, neutral sugar and amino sugar released from home submaxillary mucin by different conditions of acid hydrolysis were analyzed by trimethylsilylation and acetylation of capillary gas chromatography, respectively. The fraction of sugar in bovine submaxillary mucin was composed of 4. 3 % of N-acetylneuraminic acid, 19.0% of N-glycolylneuraminic acid, 3. 2% of fucose, 5.1 % of galactose, 18. 4% of N-acetylgalacetosamine, 8. 7 % of N- acetylglucosamine.

关 键 词：粘蛋白 单糖组成 BSM GC 分析 颌下腺粘蛋白 

分 类 号：Q513.203[生物学—生物化学]