秦岭华中五味子种子内源抑制物活性研究  

Studies on Activity of Endogenesis Inhibitory Aubstances of Schisandra sphenanthera Rehd.et Wils.Seeds Gowing in Qinling Mountain

作　　者：齐永平[1] 顾蔚[1,2,3] 罗成[1] 王喆之[1,2,3] 

机构地区：[1]陕西师范大学生命科学学院,西安710062 [2]陕西师范大学药用植物资源与天然药物化学教育部重点实验室,西安710062 [3]陕西师范大学西北濒危药材资源开发国家工程实验室,西安710062

出　　处：《种子》2009年第8期1-3,8,共4页Seed

基　　金：国家"十一五"科技支撑计划项目(2006BAI06A13-06);陕西师范大学研究生培养创新基金项目(2008CXS040)

摘　　要：研究了秦岭山区野生华中五味子种子内源抑制物存在部位及其抑制活性。以甲醇和蒸馏水分别对华中五味子种皮和种仁进行浸提,分别制备成5个不同质量浓度的粗提物,用白菜种子测定其抑制活性。结果表明,华中五味子种皮和种仁均含有能极显著抑制白菜种子萌发和幼苗生长的内源抑制物,且其抑制活性随着质量浓度升高逐渐增强。相同部位和质量浓度的甲醇粗提物的抑制活性高于蒸馏水粗提物,相同提取溶剂和质量浓度的种皮粗提物对白菜种子萌发和幼苗生长的抑制活性强于种仁。该结果表明,华中五味子种子内源抑制物的存在可能是其休眠期长、不易萌发的主要原因之一。The inhibitory activity and the location of endogenetic inhibitory substances of Rehd. et Wils. seeds were investigated in this study. The crudeextracts from different part of the seeds of S. sphenanthera were prepared using methanol and distilled water as impregnant and diluted in 5 mass concentration grads. The inhibitory activities were determined by its effect on the seeds of Brassica sp.. The result indicated that endogenetic inhibitory substances which obviously inhibited the seed germination and the plumule growth of Brassica sp. exist in the seed coat and kernel of S. sphenanthera seeds, and the inhibitory activity was positively related to the mass concentration of the crude extracts. The inhibitory activity methanol crude extracts was stronger than aqueous crude extracts when they were from the same part of the seeds and in the same mass concentration. The extracts from the seed coat could inhibit Brassica sp. seed germinating and plumule growth at a higher level than that from the kernel when they were prepared by the same impregnant and in same mass concentration. In Conclusion, both of the seed coat and kernel had highly active endogenetic inhibitory substances. This result suggested that endogenetic inhibitory substances might be the main reason of Schisandra sphenanthera Rehd. et Wils. long period dormancy.

关 键 词：华中五味子 种子 内源抑制物质 抑制活性 

分 类 号：Q949.95[生物学—植物学]