高效液相色谱法同时测定牛肉及其制品中敌草隆、绿麦隆的残留量  

The Determination of Diuron and Chlortoluron Residues in Beef and Beef Products by High Performance Liquid Chromatography

作　　者：韩红岩[1,2] 李军民 曹生君 黄化成[1,2] 

机构地区：[1]山东农业大学 [2]山东菏泽商检局

出　　处：《色谱》1998年第4期367-368,共2页Chinese Journal of Chromatography

摘　　要：介绍了用高效液相色谱同时测定牛肉及其制品中脲类除草剂——敌草隆、绿麦隆残留量的方法。色谱柱为ＳｅｌｅｃｔｏｓｉｌＣ１８柱，流动相为甲醇－水（６０∶４０，Ｖ／Ｖ），ＵＶ－２４５ｎｍ检测。最小检测量：敌草隆为０．４ｎｇ，绿麦隆为０．５ｎｇ。线性范围均为０．０５～１０ｍｇ／Ｌ。回收率：敌草隆为８７．３４％～８７．６４％，绿麦隆为８８．７８％～９１．９４％。Amethod for the determination of diuron and chlortoluron in beef and beef products with high performance liquid chromatography is described. The sample was extracted with a mixture of acetonitrile and methanol (50∶50, V/V). After filtration, the filtrate was defatted with petroleum ether, then water was added and further extracted with chloroform. The chloroform collected was evaporated in a rotary evaporator(45℃). The residue was dissolved in acetonitrile methanol(50∶50,V/V) mixture, poured into an Al 2O 3 column and eluted with the same mixture. The eluate was collected for HPLC analysis. The analytical column was Selectosil C 18 (5μm, 250mm×4 6mm i.d.), mobile phase was methanol water(60∶40, V/V) and detection wavelength was UV 245nm. The minimum amounts of detection were 0 4ng for diuron and 0 5ng for chlortoluron. Recoveries were 87 34% 87 64% for diuron and 88 78% 91 94% for chlortoluron.

关 键 词：敌草隆 绿麦隆 牛肉制品 HPLC 测定 农药残留 

分 类 号：TS207.53[轻工技术与工程—食品科学] TS251.52[轻工技术与工程—食品科学与工程]