FZS中药合剂对细胞凋亡的保护作用研究  

Mechanism of a compound Chinese traditional medicine FZS for apoptosis

作　　者：王建秀[1] 王德生[1] 段淑荣[1] 赵敬堃[1] 郭彩玲[2] 张凤民[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院神经内科,黑龙江哈尔滨150001 [2]哈尔滨医科大学基础医学院微生物学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2009年第4期320-324,共5页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(30670726);黑龙江省卫生厅资助项目(2006-008)

摘　　要：目的探讨FZS中药合剂对Aβ25-35、Aβ42、Aβ42寡聚体诱导的PC12细胞凋亡的保护作用。方法应用正常细胞进行对照研究,通过测定MTT、LDH和原位末端标记(TUNEL)方法检测细胞凋亡。原子力显微镜观察PC12细胞表面突起的变化。结果MTT在FZS组明显增高(P<0.05);LDH、TUNEL凋亡率在FZS组表达明显降低(P<0.05);原子力显微镜观察Aβ致细胞表面突起缩短的现象在FZS组明显改善,差异有显著意义(P<0.05)。结论FZS可以通过降低Aβ的细胞凋亡作用而减少其细胞毒性,FZS中药合剂可能成为Alzheimer病的治疗手段之一。AbstrObjective To investigate the protective effects of compound Chinese traditional medicine FZS to Aβ25-35,Aβ42 and Aβ42 oligomer induced apoptosis of PC12 cells. Methods Neuronal cells living were examined by MTT and LDH, while neuronal apoptosis examined by TdT-mediated dUTP-biotin nick end labeling （TUNEL） assays using a normal cell control testing. We have used atomic force microscopy （AFM） to visualize the PC12 cell surfaces. Results LDH and TUNEL were significantly decreased, while MTF was significantly increased in FZS group （ P 〈 0. 05 ）. The data showed that the surface projection of PC12 cell was shortened in Aβ group, and was significantly promoted with FZS treatment（P〈 0. 05）. Conclusion FZS may reduce toxicity of Aβ via reduction of apoptosis, which could be a novel strategy for Alzheimer＇ s disease （AD） therapeutics.

关 键 词：Β-淀粉样蛋白 寡聚体 凋亡 中药合剂 原子力显微镜 

分 类 号：R749.1[医药卫生—神经病学与精神病学]