低氧诱导下Tca8113细胞HIF-1α与VEGF的表达及血管生成相关性研究  

Expression of HIF-1α and VEGF in Tca8113 Cell under Hypoxia Microenvironment

作　　者：闵睿[1] 康非吾[1] 王开[1] 吴湣[1] 朱炎[1] 

机构地区：[1]同济大学附属口腔医院口腔颌面外科,上海200072

出　　处：《口腔颌面外科杂志》2009年第4期234-238,共5页Journal of Oral and Maxillofacial Surgery

基　　金：上海市青年科技启明星计划(09QA1406400)

摘　　要：目的:研究在体外缺氧培养条件下舌鳞癌细胞系Tca8113中缺氧诱导因子-1α(hypoxia-inducible factor 1alpha,HIF-1α)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达,探讨HIF-1α、VEGF在缺氧条件下对舌鳞癌血管生成的作用机制及相互联系。方法:CoCl2化学缺氧法模拟肿瘤缺氧环境,MTT法检测细胞生长能力。荧光定量PCR,Western blot法分别检测不同缺氧状态下HIF-1α和VEGF在mRNA和蛋白水平的表达并进行相关性研究。结果:MTT发现CoCl2诱导的缺氧对细胞的生长起抑制作用。低氧条件下,Tca8113细胞HIF-1αmRNA水平稳定,蛋白表达显著升高,而VEGF mRNA和蛋白的表达均显著升高。结论:CoCl2诱导的缺氧使Tca8113细胞HIF-1α在蛋白水平表达升高,并通过转录激活VEGF的表达,调控舌鳞癌的血管生成。Objective： To investigate the expression of HIF-1α and VEGF in human tongue squamous cancer cell line Tca8113 under hypoxia. To observe the effect of HIF-1α on hypoxia-activatad angiogenesis regulation pathway in squamous cell carcinoma （SCC）. Methods： COCl2 was used as a chemical hypoxia -inducible reagent to mimic tumor hypoxic microenvironment, mRNA and protein levels of HIF-1α and VEGF were detected by real-time fluorescent PCR. Western-blotting and real-time fluorescent PCR were used to observe the change of HIF-1α and VEGF gene protein expression under hypoxia.The effect of HIF-1α on cell proliferation was estimated by MTT assay. Results： Under hypoxia, mRNA level of HIF-1α was stable, while its protein level increased obviously. Both mRNA and protein levels of VEGF were up-regulated. The hypoxia induced by COCl2 had a prohibitive effect on cell proliferation. Conclusion： Hypoxia microenvironment could induce the over-expression of HIF-1α in human oral squamous cell carcinoma cell line Tca8113. There was a time-dependent and quantity-dependent response under some circumstances. HIF-1α up-regulated the gene expression of VEGF which promotes angiogenesis in SCC under hypoxia microenvironment.

关 键 词：缺氧诱导因子-1Α 缺氧 血管内皮生长因子 荧光PCR 二氯化钴 

分 类 号：R739.8[医药卫生—肿瘤]