突变型Nbs1基因转染对头颈癌化疗敏感性的影响  

Chemo-sensitivity of Cancer Cells of the Head and Neck after Transfected with Mutant Nbs1 Gene

作　　者：王代友[1] 巫家晓[1] 张斌[1] 陆新萍[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,广西南宁530021

出　　处：《口腔颌面外科杂志》2009年第4期239-242,共4页Journal of Oral and Maxillofacial Surgery

基　　金：广西自然科学基金资助(桂科自0640095)

摘　　要：目的:探讨突变型Nbs1基因转染对头颈癌细胞化疗敏感性的影响。方法:用突变型Nbs1腺病毒载体转染头颈癌细胞株JHU006,同时给予顺铂处理,24 h后,用MTT法测定癌细胞生长曲线,中性彗星法检测癌细胞DNA双链断裂程度。结果:JHU006细胞株在转染突变型Nbs1基因加顺铂治疗后,细胞生长处于停滞状态,至第6天大部分细胞死亡;中性彗星实验显示,该组癌细胞平均尾力矩值明显增高。结论:突变型Nbs1基因转染可显著抑制头颈癌细胞生长,并阻断了DNA双链断裂损伤的修复,因而增加了头颈癌细胞的化疗敏感性。Objective： To determine the chemo-sensitivity of head and neck cancer cells after transfected with mutant Nbsl （Nijmegen breakage syndrome 1 ） gene. Methods： JHU006 cells were transfected with mutant Nbsl and got chemotherapy with cisplatin for 24 hours. MTT method was employed to determine the growth curve for head and neck cancer ceils JHU006. Neutral comet assay was used for observing the cells＇ DNA double strain breaks. Results： After the treatment, JHU006 cells almost didn＇t grow any more and their values of the mean tail moment remarkably rose. Conclusion： Transfected with mutant Nbsl gene, head and neck cancer cells＇ chemo-sensitivity significantly increased attribute to the inhibition of repairs to DNA double strain breaks.

关 键 词：Nbs1基因 化疗敏感性 头颈癌 

分 类 号：R739.91[医药卫生—肿瘤]