老年胃癌组织中P16,P53蛋白和增殖细胞核抗原表达的研究  

Research on expressions of P16 and P53 proteins and PCNA in elderly patients with gastric cancer

作　　者：常东民[1] 马刚[1] 张勇[1] 王作仁[2] 姬生威[1] 王伯良[3] 

机构地区：[1]西安交通大学第一附属医院肿瘤外科,陕西西安710061 [2]西安交通大学第一附属医院肝胆外科,陕西西安710061 [3]第四军医大学唐都医院急诊科,陕西西安710038

出　　处：《第四军医大学学报》2009年第16期1468-1471,共4页Journal of the Fourth Military Medical University

基　　金：陕西省科技攻关项目(2008K09-3)

摘　　要：目的:研究老年胃癌组织中P16,P53蛋白和增殖细胞核抗原(PCNA)在胃癌的发生发展中表达并讨论其临床意义.方法:应用免疫组织化学方法,对57例胃癌组织和癌旁黏膜组织、19例胃正常组织中P16,P53蛋白表达产物和PCNA进行检测,并结合临床进行讨论和分析.结果:老年患者胃癌组织中P16蛋白的阳性率为21%(12/57),明显低于癌旁黏膜组织60%(34/57)和胃正常组织89%(17/19,P<0.05);P53蛋白与PCNA在胃癌组织中的阳性率为81%(46/57)和93%(53/57),明显高于癌旁黏膜组织42%(24/57),61%(35/57)和胃正常组织0%(0/19,P<0.05).P16,P53蛋白和PCNA阳性表达与胃癌组织学类型、浸润深度、分化程度及淋巴结转移有显著差异(P<0.05),与患者年龄、性别、肿瘤大小及部位无关(P>0.05).结论:老年胃患者P16,P53蛋白和PCNA的异常表达在胃癌的发生发展、恶性程度、淋巴结转移及预后中起一定作用,研究他们的动态变化有重要的临床意义.AIM： To study the expressions of P16 and P53 proteins and proliferating cell nuclear antigen （PCNA） in the occurrence and development in elderly patients with gastric cancer. METHODS： P16 and P53 proteins as well as PCNA expressions were detected in 57 specimens of gastric cancer and pericarcinoma tissues, and 19 samples of normal gastric tissues by immunohistochemistry. The data were analyzed together with clinical manifestations. RESULTS： The positive rate of P16 protein expression was 21% （12/57） in gastric cancer samples, significantly lower than that in both pericarcinoma tissues 60% （34/57） and normal gastric tissues 89% （17/19,P 〈 0.05 ）. The positive rates of P53 protein and PCNA expression were 81% （46/57） and 93% （53/ 57） in gastric cancer samples, significantly higher than those in pericarcinoma tissues （42% , 61% ） and normal gastric tissues （0%, P〈0.05）. The positive expressions of the P16 and P53 proteins and PCNA were correlated with the histological types and degrees of differentiation of gastric cancer and the depth of invasion and lymph node metastasis （ P 〈 0.05 ）, but were not correlated with the age, sex, tumor size and sites （ P 〉 0.05 ）. CONCLUSION： Abnormal expressions of P16 and P53 proteins, and PCNA are closely correlated to the occurrence, development, malignancy degrees, lymph node metastasis and prognosis of gastric cancer. The detection of P16, P53 and PCNA is of important clinical significance for gastric cancer therapy.

关 键 词：胃肿瘤 老年人 病人 蛋白质P16 蛋白质P53 增殖细胞核抗原 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]