选择性COX-2抑制剂抑制肺癌生长的实验研究  

Experimental study on inhibitive effect of celecoxib on lung cancer

作　　者：周丹[1] 陈鸿义[2] 刘桐林[2] 

机构地区：[1]武汉亚洲心脏病医院心胸外科,湖北武汉430022 [2]北京大学第一医院胸外科,北京100034

出　　处：《第四军医大学学报》2009年第16期1472-1475,共4页Journal of the Fourth Military Medical University

摘　　要：目的:观察选择性环氧合酶-2(COX-2)抑制剂塞来昔布对肺癌A549细胞在体内外的抑制作用.方法:噻唑蓝(MTT)比色法检测塞来昔布对肺癌细胞的增殖抑制,流式细胞技术检测药物处理后A549的细胞周期,观察塞来昔布对裸鼠A549细胞移植瘤的抑制作用,移植瘤切片TUNEL法检测癌细胞凋亡.结果:塞来昔布对肺癌细胞增殖有抑制作用,IC50为50μmmol/L;阻滞G1期细胞进入S期.称量裸鼠移植肿瘤的瘤结节质量,对照组(2.16±0.98)g,药物组(0.98±0.51)g,两组差异有统计学意义(P<0.05),抑瘤率为54.6%.TUNEL法移植瘤切片凋亡细胞染色对照组凋亡指数(AI)为1.6±0.6,用药组为9.5±2.5,差异有统计学意义(P<0.05).结论:塞来昔布在体内及体外均对肺癌细胞表现有抑制作用,将细胞周期阻滞在G1期并诱导肿瘤细胞凋亡可能是抑制肺癌细胞增殖的重要机制.AIM： To observe the inhibitive effect of celecoxib, a selective inhibitor of cyclooxygenasc-2 （ COX-2 ）, in vitro and in vivo, on the proliferation of A549 cell line. METHODS： Antiproliferative effect was detected by MTT assay and flow cytometry was used to analyze the effect of celecoxib on cell cycles. Nude mice bearing lung cancer xenografts were treated with celecoxib and then the weight of implanted tumors was measured. The apoptotic cells in implanted tumors were stained by terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling （TUNEL） technique. RESULTS ： Celecoxib inhibited the cell proliferation of lung cancer cells in a dose-dependent manner and IC50 was 50 umoL/L. Celecoxib induced the cycle arrest at G0/G1 stage, inhibiting the transition of G1 cells to S phrase. The average weight of tumor nodules in control group was （2.16 ± 0.98 ） g, while in the experimental group the average weight was （0.98 ±0.51 ）g, with the inhibitive rate of 54.6% （P 〈 0.05）. Apoptosis of tumor cells showed that apoptotic index （AI） was 1.6±0.6 in the control group and 9.5±2.5 in the experimental group, with significant differences （P 〈 0. 05 ）. CONCLUSION： Celecoxib may exert its suppressive effect in vitro and in vivo on the proliferation of A549 cell line by inducing cell cycle arrest at G0-G1 stage of A549 cell and apoptosis of the tumor cells.

关 键 词：肺肿瘤 环氧合酶-2 塞来昔布 细胞周期 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]