阿司匹林对萘性白内障氧化损伤的抑制作用  

Experimental study of low-dose aspirin in preventing naphthalene-induced cataract in rats

作　　者：陈燕[1] 卢奕[1] 邱斌[1] 蒋永祥[1] 笪翠弟[1] 

机构地区：[1]复旦大学附属眼耳鼻喉医院眼科,上海200031

出　　处：《眼视光学杂志》2009年第4期269-272,共4页Chinese Journal of Optometry & Ophthalmology

基　　金：上海市科学技术委员会资助项目(08411963300)

摘　　要：目的探讨低剂量阿司匹林对萘性白内障氧化损伤的抑制作用及其作用机制。方法将45只50～160g雌性SD大鼠随机分为三组,每组15只:空白对照组、萘性白内障组[0.5mg/(kg·d)萘灌胃3d后改为1mg/(kg·d)萘灌胃]、阿司匹林组[萘灌胃4h后用100mg/(kg·d)阿司匹林灌胃]。每周使用计算机灰度分析系统进行图像混浊程度分析比较,于第9周末取大鼠晶状体,检测晶状体匀浆谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)水平及羟自由基抑制能力等生化指标,采用完全随机方差分析(One Way ANOV)比较各组间灰度值及生化指标。结果第3周时,萘组晶状体开始出现混浊,程度逐渐加重;而阿司匹林组在早期晶状体混浊进展较萘组慢,第6周后接近于萘组,两组的混浊程度均明显高于空白对照组。阿司匹林组晶状体匀浆的GSH量、GSH-PX活性及羟自由基抑制能力均高于萘组,而晶状体匀浆的MDA量低于萘组。结论阿司匹林可通过抑制氧化损伤,延缓萘性白内障大鼠晶状体混浊的进展,这种延缓作用在萘性白内障早期尤其明显。Objective To investigate the role of low-dose aspirin in preventing naphthalene-induced cataract. Methods Forty-five 150-160 g female SD rats were randomly divided into 3 groups： a control group （without any treatment）, a naphthalene -induced cataract group [given 0.5 mg/（kg·d） orally for 3 days then 1 mg/ （kg·d） on the following days], and an aspirin treatment group [naphthalene-induced cataract rats given 100 mg/（kg·d） of aspirin orally]. The progression of lens opacifieation was recorded at regular time intervals using a slit lamp. The levels of GSH, GSH-PX, MDA and the inhibiting ability of OH- were examined. Results Opaeifieation of the naphthalene-induced cataract gradually developed after 3 weeks. In the aspirin treatment group, it developed slowly during the first 6 weeks, but progressed quickly from then on. Compared to the naphthalene-induced cataract group, the levels of GSH, GSH-PX and the inhibiting ability of OH- in the aspirin treatment group were significantly higher and the level of MDA was significantly lower in the aspirin treatment group. Conclusion Aspirin can delay the progression of lens opacification. Its function is apparent in the earlier stages. The delay may be associated with the ability of aspirin to protect against oxidative damage.

关 键 词：萘性白内障 阿司匹林 氧化损伤 

分 类 号：R779.1[医药卫生—眼科]