顺铂对bcr/abl转化BaF3-P210细胞DNA损伤与修复能力的影响  

The effect of cisplatin on the DNA injury and repair ability of bcr-abl-transformed BaF3-P210 cells

作　　者：朱喜丹[1] 史梦[1] 袁颖[2] 曾建明[1] 黄世峰[1] 陶崑[1] 冯文莉[1] 

机构地区：[1]重庆医科大学临床血液学教研室临床检验诊断学教育部重点实验室,重庆400016 [2]重庆医科大学临床医学系,重庆400016

出　　处：《肿瘤》2009年第8期731-735,共5页Tumor

基　　金：教育部高等学校博士学科点专项科研基金(编号:20050631007)

摘　　要：目的:研究顺铂(cisplatin,Cis)对白血病BaF3-MIGR1细胞和bcr/abl转化BaF3-P210细胞中DNA损伤修复能力的影响。方法:锥虫蓝染色法检测2种细胞经0.5、1、5、10、20和40μg/mL Cis处理0、2、4、6、8、12、24和48 h时的存活率,免疫荧光法检测上述6种浓度Cis处理细胞8 h后诱导的γH2AX焦点数,Western印迹法检测DNA修复0、4、12和24 h后的γH2AX蛋白表达情况。结果:随着Cis处理时间和剂量增加,2种细胞的存活率降低,BaF3-P210转化细胞的存活率比BaF3-MIGR1细胞高(P<0.05);随着Cis剂量上升,2种细胞的γH2AX焦点数上升,BaF3-P210转化细胞的焦点数比BaF3-MIGR1细胞少(P<0.05);随着DNA修复时间延长,2种细胞的γH2AX蛋白表达均下调,BaF3-P210转化细胞的γH2AX蛋白表达量比BaF3-MIGR1细胞低。结论:Cis对BaF3-MIGR1细胞和BaF3-P210转化细胞的损伤呈时间、剂量效应,2种细胞的γH2AX焦点数与损伤之间存在剂量效应,bcr/abl可增强BaF3-P210转化细胞的DNA修复能力。Objective： To investigate the effect of cisplatin（Cis） on the DNA injury and repair ability of leukemia BaF3-MIGR1 cells and bcr-abl-transformed BaF3-P210 cells.Methods： The viabilities of two cell lines were evaluated by trypan blue test at 0,2,4,6,8,12,24,and 48 h after treated with Cis at 0.5,1,5,10,20,and 40 μg/mL.The number of γH2AX foci was assessed by immunofluorescent microscopy after cells were treated with 6 concentrations of Cis for 8 h.Western blotting was employed to determine the expression of γH2AX protein in the two cell lines after 0-,4-,12-,and 24-h DNA repairment.Results：The viabilities of the two groups of cells were decreased by Cis in a time-and dose-dependent manner.The surviving rate of BaF3-P210 cells was higher than that of the BaF3-MIGR1cells（P〈0.05）.Immunofluorescent microscopy indicated that the number of γH2AX foci in the two cell lines was increased with elevated Cis dosages,and furthermore,the number of γH2AX foci in BaF3-P210 transformed cells was less than that in BaF3-MIGR1 cells（P〈0.05）.The protein expression of γH2AX in two cell lines was down-regulated with the prolongation of DNA repair time.The γH2AX protein expression in BaF3-P210 transformed cells was lower than that in BaF3-MIGR1 cells.Conclusion： Cis exhibits a dose-and time-dependent cytotoxic effect on BaF3-MIGR1 cells and BaF3-P210 transformed cells.Cis induces a dose-dependent increase in the number of γH2AX foci in the two cell lines.Bcr/abl fusion protein could increases the DNA repair ability of BaF3-P210 transformed cells.

关 键 词：白血病 髓样 慢性 顺铂 γH2AX蛋白质 DNA损伤 DNA修复 融合蛋白质类 bcr-abl 

分 类 号：R733.7[医药卫生—肿瘤]