次级淋巴组织趋化因子对溃疡性结肠炎大鼠淋巴细胞的体外趋化作用  

Migration of ulcerative colitis lymphocytes in vitro induced by secondary lymphoid tissue chemokine

作　　者：葛步军[1] 陈锡美[2] 杨长青[2] 吴剑[2] 

机构地区：[1]同济大学附属同济医院外科,上海200065 [2]同济大学附属同济医院消化科,上海200065

出　　处：《中华实验外科杂志》2009年第9期1176-1178,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30470794）

摘　　要：目的观察次级淋巴组织趋化因子（SLC）对溃疡性结肠炎（UC）大鼠淋巴细胞的体外迁移作用。方法采取UC模型组、对照组大鼠各20只大鼠的门静脉血，分离出淋巴细胞培养，通过流式细胞仪检测淋巴细胞表达趋化因子受体CCR7，利用Boyden小室法探讨趋化因子SLC对不同组别淋巴细胞的体外趋化作用．结果流式细胞仪结果证实二组大鼠淋巴细胞均能表达趋化因子受体CCR7，模型组结肠静脉回流血中CCR7^＋淋巴细胞的比例（68．74±5．18）％明显高于对照组（16．62±7．84）％，P〈0．01，SLC可增强淋巴细胞的移行能力，SLC对模型组淋巴细胞的趋化活性明显高于对照组，并呈现剂量效应关系，80ug/L为其饱和浓度。结论SLC／CCR7可促进淋巴细胞的迁移，为探讨UC肠淋巴细胞归巢提供实验依据。Objective To explore the role of lymphocytes migration induced by secondary lymphoid tissue chemokine （SLC） in rats with ulcerative colitis. Methods Forty SD rats were randomly divided into control and model groups equally. The portal blood was collected, lymphocytes were isolated to culture, chemokine receptor CCR7 was detected by flow cytometry, and the lymphocytes migration in vitro induced by SLC in different groups were explored by using Boyden chamber. Results Flow cytometry revealed that CCR7^＋ were expressed in lymphocytes of two groups, and the proportion of CCR7^＋ lymphocytes in model group （68.74±5.18） was higher than that in control group （ 16.62±7.84,P 〈0. 01 ） significantly. The chemotactic activity of model lymphocytes induced by SLC was stronger than that of control group significantly, and migration capabilities of lymphocytes could be enhanced by SLC in a concentrationdependent manner within a certain concentration. 80 ug/L of SLC was the saturated dose. Conclusion SLC/CCR7 can promote lymphocytes migration, which provides a theoretical basis to study intestinal lymphocyte homing.

关 键 词：溃疡性结肠炎 淋巴细胞 趋化因子 流式细胞法 

分 类 号：R574.62[医药卫生—消化系统]