大鼠胰腺癌和非癌胰腺组织中胰岛素样生长因子及其受体的表达  

Expression of iusulin.like growth factor-1 （ IGF-1 ） and its receptor （IGF-1R） and significance in pancreatic cancer and non-cancerous pancreatic tissues of Sprague-Dawely rats

作　　者：范文涛[1] 杨竹林[1] 刘洁琼[1] 杨乐平[1] 苗雄鹰[1] 

机构地区：[1]中南大学湘雅二医院肝胆疾病研究室,长沙410011

出　　处：《中华实验外科杂志》2009年第9期1179-1181,F0004,共4页Chinese Journal of Experimental Surgery

摘　　要：目的建立SD大鼠胰腺癌模型，探讨胰腺癌发生过程中胰岛素样生长因子（IGF）-1和其受体（IGF-1R）的表达及其意义。方法将二甲基苯葬蒽（DMBA）直接置入胰腺实质内（A组＋B组），B组每周腹腔注射曲古霉素A（TSA）1ug；A组和B组于3～5个月内处死，对照组（C组）于第5个月处死；肉眼检查和HE染色后镜下观察胰腺癌发生；IGF-1和IGF-1R染色方法为常规石蜡包埋切片EnVision^TM免疫组织化学法。结果A组3～5个月癌发生率为48．7％（18／37），17例为胰腺导管癌和1例为纤维肉瘤；B组3—5个月癌发生率为33．3％（12／36），11例为胰腺导管癌和1例为纤维肉瘤；A组胰腺癌最大径均值大于B组（P〈0．05）；C组胰腺及A、B、C组胰腺外主要脏器均无明显病理改变；A组＋B组胰腺导管癌IGF-1和IGF-1R表达阳性率明显高于A组＋B组非癌胰腺组织（P〈0．01），A组胰腺导管癌IGF-1和IGF-1R表达阳性率明显高于A组非癌胰腺组织（P〈0．01），但B组之间差异无统计学意义（P〉0．05）；IGF-1和IGF-1R阳性表达的非癌胰腺导管上皮呈轻至重度不典型增生；C组正常胰腺和2例纤维肉瘤IGF-1和IGF-1R均阴性表达。结论较大剂量DMBA置入胰实质内可在短期内获得较高胰腺癌发生率，TSA能抑制胰腺癌的生长，其作用可能与抑制IGF-1和IGF-1R合成和分泌有关。IGF-1和IGF-1R在DMBA诱癌发生过程中可能起着重要促癌作用。Objective To establish a model of pancreatic cancer induced by dimethylbenzanthracene （DMBA） in Spragne-Dawely （SD） rats, and detect the expression levels of insulin-like growth factor- 1 （ IGF-1 ） and its receptor （ IGF-1 R） and their effects on the carcinogenesis of rat pancreas. Methods DMBA was directly implanted into the parenchyma of rat pancreas （ group A and group B ）. The rats in group B were treated with 1 ml trichostatin A （TSA） saline solution （1 mg/L） via intraperitoneally weekly. The carcinogenesis of rats executed within 3-5 months in groups A and group B was observed by macrograph and under microscopy. Meanwhile, the rats in the control group （ group C ） were executed in 5 months. The EnVisionTM immunohistochemistry for detecting the expression levels of IGF-1 and IGF-1R was used in conventionally paraffin-embedded sections from above pancreatic specimens. Results （ 1 ） The incidence of pancreatic cancer in group A within 3-5 months was 48.7% （ 18/37 ） ,including 17 cases of pancreatic ductal adenocarcinoma and 1 case of fibrosarcoma. The incidence of pancreatic cancer in group B within 3-5 months was 33.3% （ 12/36 ） , including 11 cases of pancreatic duetal adenocarcinoma and 1 case of fibrosareoma. The maximal diameter of mass was larger in group A than in group B （ P 〈 0.05）. No pathological changes were found in pancreas of group C and other main organs of all groups. （2） The positive expression rate of IGF-1 and IGF-1R in groups A and B was significiantly higher in ductal adenocarcinoma than in non-cancerous pancreatic tissues （ P 〈 0.01 ）. The positive expression rate of IGF-1 and IGF-1R in group A was significantly higher in ductal adenocarcinoma than in non-cancerous pancreatic tissues （ P 〈0.05 ）, but no statistically significant difference was found in group B （ P 〉0.05 ）. The positive cases of IGF-1 and/or IGF-1R in non-cancerous pancreatic tissues showed mild-to-severe atypical hyperplasia of ductal epithelia. Panc

关 键 词：胰腺肿瘤 胰岛素样生长因子-1 

分 类 号：R735.9[医药卫生—肿瘤]