应用硒酸精氨酸治疗酒精性肝损伤小鼠的初步研究  

Preliminary study of selenoarginine (SeArg) in treatment of alcohol-induced liver injury in mice

作　　者：张国蓉[1] 孙海波[1] 刘安军[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中国现代医学杂志》2009年第16期2438-2440,2445,共4页China Journal of Modern Medicine

基　　金：天津科技大学人才引进及科研启动基金(0200038)

摘　　要：目的研究硒酸精氨酸对小鼠酒精性肝损伤的保护作用。方法用连续灌服酒精的方法建立小鼠酒精性肝损伤模型。将60只雄性小鼠随机分为4组:对照组、模型组、硒酸精氨酸处理组和二氧化硒处理组。连续灌胃8周,测定小鼠体重的变化;实验结束后,计算小鼠肝指数和脾指数;取小鼠肝脏制成肝匀浆,测定其中的丙二醛(MDA)、蛋白质羰基(PC)的含量;取小鼠血清测定肿瘤坏死因子(TNF-α)水平。结果硒酸精氨酸能显著降低酒精性肝损伤引起的MDA、PC和TNF-α的升高(P<0.01),硒酸精氨酸对肝脏的保护作用明显优于二氧化硒。结论硒酸精氨酸对小鼠酒精性肝损伤具有显著的保护作用,效果也优于二氧化硒。[Objective ] To study the protective effect of selenoarginine against alcohol-induced liver injury in mice. [ Methods ] Alcohol-induced liver injury mouse model was set up by executive intragastric administration. 60 male mice were randomly divided into four groups： control group, model group, selenoarginine group and SeO2 group. Mice were constitute i.g. for 8 weeks, mice body weight changes were measured; Calculated mice liver index and spleen index when experiment finished; Liver homogenate were made from mice, contents of MDA, PC, TNF-α were measured. [ Results] Selenoarginine significantly reduced the elevation of MDA, PC, TNF-α（P 〈0.01） caused by alcohol, and there was better protective effect of selenoarginine compared with SeO2. [ Conclusion ] Selenoarginine had an obviously protective effect against alcohol-induced liver injury, and performed better than SeO2.

关 键 词：酒精性肝损伤 硒酸精氨酸 二氧化硒 肿瘤坏死因子 

分 类 号：R-332[医药卫生]