苦参碱对唾液腺腺样囊性癌细胞黏附侵袭抑制的影响及机制探讨  

Effect and mechanism of matrine on adhesion and invasion of salivary gland adenoid cystic carcinoma cells in vitro

作　　者：赵军方[1] 李新明[1] 谢卫红[1] 王海斌[1] 方政[1] 李青川[1] 

机构地区：[1]郑州大学第一附属医院口腔颌面外科,河南郑州450052

出　　处：《上海口腔医学》2009年第4期401-405,共5页Shanghai Journal of Stomatology

基　　金：河南省教育厅自然科学基金(2008A320035)~~

摘　　要：目的:研究苦参碱对唾液腺腺样囊性癌ACC-M细胞黏附侵袭能力的影响并初步探讨其机制。方法:体外培养ACC-M细胞系,MTT法测定药物对细胞增殖的抑制作用,应用黏附试验和Matrigel侵袭实验,研究苦参碱注射液及其不同浓度对ACC-M细胞黏附及侵袭能力的影响,采用SPSS11.0软件包对数据进行t检验。结果:苦参碱注射液浓度在0.25、0.50、0.75、1.00 mg/mL时,对ACC-M细胞均有一定增殖抑制作用,且呈明显的量效和时效关系。不同浓度苦参碱注射液处理ACC-M细胞24h后,其体外黏附和侵袭能力呈剂量依赖性下降。经苦参碱(0.75 mg/mL)作用后,ACC-M细胞的E钙黏素(E-cad蛋白)表达明显增强,与对照组有显著差异。结论:一定浓度的苦参碱可以抑制唾液腺腺样囊性癌细胞生长,降低细胞黏附和侵袭能力,其机制可能与上调E-cad蛋白表达有关。PURPOSE：To investigate the effect and mechanism of matrine on adhesion and invasion of human adenoid cystic carcinoma ACC-M cells in vitro.METHODS：MTT assay was used to examine the effect of matrine on proliferation of ACC-M cells treated after 1 day to 3 days.Byden chamber assay was performed to detect the effect of matrine on invasion capacity of the cells;Effect on adhesion potential of ACC-M cells was tested by cell matrigel adhesion assay.Mechanism of inhibitory adhesion and invasion was investigated by expression of E-cad protein.The results was analyzed by SPSS11.0 software package.RESULTS：Matrine could inhibit the proliferation of ACC-M cell lines with apparent dose-dependent and time-dependent effect.Matrine significantly inhibited adhesion and invasion capacity of ACC-M cell lines in vitro with concentration increasing.The expression level of E-cad was significantly increased compared with the untreated group in ACC-M cell lines.CONCLUSIONS：Matrine can inhibit the adhesion and invasion capacity of ACC-M cell lines in vitro.Mechanism of the inhibition effect may be related to over expression of E-cad protein.

关 键 词：唾液腺腺样囊性癌 黏附 侵袭 苦参碱 E-CAD 

分 类 号：R739.8[医药卫生—肿瘤]