超短波对脑缺血大鼠治疗时机的研究  

Study on Secure Time of Ultroshortwave Therapy in Brain Ischemia of Rat

作　　者：孙锋[1] 王金春[1] 闻霞[1] 

机构地区：[1]辽宁省沈阳市第五人民医院神经内科,110023

出　　处：《中国误诊学杂志》2009年第25期6055-6057,共3页Chinese Journal of Misdiagnostics

摘　　要：目的:探索超短波疗法对缺血性脑血管病的安全治疗时机。方法:采用栓线法制备大鼠局灶性脑缺血模型。选择健康Wister大鼠90只,随机分为假手术组、模型组、治疗A组及治疗B组。分别选择再灌注后的3 h及8 h为治疗A组和治疗B组的治疗时间点。通过比较各组脑梗死体积及Bcl-2及TNF-α含量的不同。结果:再灌注8h时给予超短波治疗能减小大鼠的脑梗死体积,增加Bcl-2的阳性表达细胞,减少TNF-α的阳性表达细胞,从而减轻再灌注损伤。而再灌注3 h时给予超短波治疗则能增大梗死体积,减少Bcl-2的阳性表达细胞,增加TNF-α的阳性表达细胞,从而加重再灌注损伤。结论:超短波疗法虽然能改善脑血流,在缺血性脑血管病中应用很广泛,但此疗法不适合在脑缺血再灌注后的超早期内应用。Objective：To study the problen of secure time on ultroshortwave therapy after ischemia reperfusion. Methods：To choose 90 healthy Wister rats and produce middle cerebral artery occlusion （MCAO） by Zea-Longa method. The 90 rats were divided into the sham operation group, model group,group A and group B randomly . To choose seperately 3 h and 8h after ischemia reperfusion as the time point of group A and group B and compare the infarct volume and determine the number of Bcl-2 and TNF-a berween the 4 groups,then research the saft time of therapy on ultrashortwave. Results： Given ultrashortwave at 3h after reperfusion could increase the infarct volume, worsen the patholigical damage ,reduce the number of Bcl-2 and raise the number of TNF-α. But the result of given the same therapy at 8h is opposite,that was,the patholigical damage was lighten. Conclusion ：Ultra short wave can arise the cerebral blood stream,so it is used greatly in the clinic work. But it can not be used in the ultra early time after ischemia-reperfusion according to the result of the test.

关 键 词：短波疗法 脑缺血/治疗 大鼠 WISTAR 随机分配 疾病模型 动物 

分 类 号：R743.31[医药卫生—神经病学与精神病学]