Ghrelin对大鼠胃黏膜上皮细胞中H^＋ -K^＋ -ATP酶基因表达和活性的影响  

Effect of Ghrelin on H^+-K^+-ATPase mRNA Expression and Activity in Gastric Mucosal Cells in Rat

作　　者：杜改梅[1] 刘茂军[2] 罗碧平[1] 蒋加进[1] 胡志华[1] 戴鼎震[1] 陈钟鸣[1] 

机构地区：[1]金陵科技学院动物科学与技术学院,江苏南京210038 [2]江苏省农业科学院兽医研究所,农业部动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《江苏农业学报》2009年第4期803-806,共4页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省自然科学基金项目(BK2007536);南京市科技发展计划项目(2008lb311085);2008年江苏省青蓝工程

摘　　要：为揭示Ghrelin对胃酸分泌的作用,从断奶大鼠中分离新鲜胃黏膜,培养胃黏膜上皮细胞,培养30 h后,试验组培养液更换为分别含有1×10-4μmol/L、1×10-3μmol/L、1×10-2μmol/L和1×10-1μmol/L Ghrelin的新鲜培养液,对照组换为不含Ghrelin的正常新鲜培养液。继续培养4 h后,收集培养液和细胞,分别测定细胞中H+-K+-ATPasemRNA表达和活性。结果表明:1×10-3μmol/LGhrelin可显著增加细胞中H+-K+-ATPasemRNA的表达(P<0.05),1×10-4μmol/L、1×10-3μmol/L和1×10-2μmol/L Ghrelin明显增加了胃黏膜上皮细胞中H+-K+-ATPase的活性。这表明Ghrelin体外作用于胃黏膜上皮细胞可刺激胃酸的分泌。To investigate the effect of ghrelin on gastric acid secretion, gastric mucosa was obtained from weaning rats. Gastric mucosal cells were dispersed from freshly gastric mucosa and exposed for 4 h to 1×10^-4 μmol/L, 1×10^-3 μmol/L, 1×10^-2 μmol/L, 1×10^- 1 μmol/L ghrelin, respectively after 30 h culture. Culture medium and cells were collected for the determination of mRNA expression and activity of H^＋ -K^＋ -ATPase. The results showed that ghrelin significantly augmented H^＋ -K^＋ -ATPase mRNA expression at 1×10^-3 μmol/L,and increased H^＋ -K^＋ -ATPase activity of gastric mucosal cells at 1 × 10^-4 μmol/L, 1×10^-3 μmol/L and 1×10^-2 μmol/L in vitro. The results indicated that ghrelin acts on gastric mucosal cells to stimulate gastric acid secretion.

关 键 词：GHRELIN 胃黏膜上皮细胞 大鼠 H+-K+-ATPase 

分 类 号：R573[医药卫生—消化系统]