兔角膜内皮细胞Na^+-K^+-ATP酶活性检测及其意义  

Assessment on Activity of Na^+-K^+-Adenosine Triphosphatase in Rabbit Corneal Endothelial Cells

作　　者：刘蓉[1,2] 张林[3] 段惠萍 

机构地区：[1]山西大同大学医学院眼科,037008 [2]西安交通大学医学院眼科 [3]西安交通大学第一附属医院眼科 [4]山西省汾阳医院眼科,032200

出　　处：《中国误诊学杂志》2009年第8期1774-1776,共3页Chinese Journal of Misdiagnostics

摘　　要：目的:探讨角膜内皮细胞上Na+-K+-ATP酶的活性。方法:运用接膜联合组织块法获得原代及传代培养的兔角膜内皮细胞(RCECs),使用考马斯亮蓝试剂盒及超微量ATP酶测试盒分别测定即兴揭取组、原代7 d组、传二代5 d组的Na+-K+-ATP酶活性。结果:即兴揭取组Na+-K+-ATP酶活性平均为(0.595±0.010)U/mgprot、原代7 d组及传二代5 d组分别为(0.572±0.007)U/mgprot和(0.333±0.011)U/mgprot,任意两两组间差异均具有统计学意义(P<0.05)。结论:原代RCECs更接近在体细胞。Na+-K+-ATP酶活性定量检测,可作为评价体外培养RCECs的功能指标。Objective：To evaluate the activity of Na^＋-K^＋ATPase in rabbit corneal endothelial cells（RCECs）.Methods：Cultured RCECs were obtained by a reliable culture method-tearing Descemet′s membrane with corneal endothelial sheet in vitro and identified by immunohistochemical staining.All cultures were divided into two teams：one was primary RCECs cultured for 7 days and achieving confluency,the other was the 2th passage cultured for 5 days and achieving confluency.The last study group was corneal endothelial cells layer acutely teared. Na^＋-K^＋-ATPase activity of all three study groups was evaluated quantitately. Results ：The Na^＋-K^＋-ATPase activity of corneal endothelial cells layer group acutely teared was （0. 595±0. 010） U/mgprot. The activity of primary RCECs was（ 0. 572±0. 007） U/mg-prot and that of the 2th passage was reduced to （0. 333±0. 011） U/mg prot. There was statistic significance between them （P〈0.05）. Conclusion：The assessment of Na^＋-K^＋-ATPase activity can be evaluated the function of the RCECs in vitro. Primary RCECs are much similar to the cells in vivo than the passages.

关 键 词：内皮 角膜/细胞学 内皮细胞/酶学 Na(+)K(+)交换ATP酶/生理学 细胞 培养的 兔 动物 

分 类 号：R772.2[医药卫生—眼科]