农残检测用木本植物酯酶的筛选研究  

Selection of Woody Plant Phytoesterases Used in the Detection of Pesticide Residues

作　　者：穆随选[1] 文建雷[2] 杨祥[2] 张檀[1] 雷明[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北林学院学报》2009年第5期135-139,共5页Journal of Northwest Forestry University

基　　金：西安市科技局攻关项目"农产品安全快速检测胆碱酯酶试剂的研制"(GG06140)

摘　　要：基于酶抑制原理,针对木本植物种子,利用总酯酶活力及比活力进行比较,筛选农药残留检测适宜的植物酯酶,扩大了植物用酯酶的选材范围,并测定了对6种有机磷及氨基甲酸酯类农药的最低检测限(LOD)。试验显示,刺槐总酯酶活力及比活力高于传统酶源小麦及其他树种,最低检测限低于国际规定的最大残留限量(MRL),因此,该酶可作为新的有机磷残留检测用酶。The seeds of woody plant were used to select phytoesterase suitable for the detection of pestiside resrdue by comparing general specific activities of the phytoesterases. The limit of detection （LOD） of 6 organophosphorus and carbamate pesticides were determined. The results showed that general and the specific esterases activities of Robinia pseudoacacia were higher than those of wheat, the traditional enzyme source; and its LOD was lower than the maximum of residue limit, meeting the international standard of detection. The enzyme can be used as a new material for the detection of the organophosphorus residues.

关 键 词：植物酯酶 有机磷农药 氨基甲酸酯农药 最低检测限 最大残留量 

分 类 号：Q503.55[生物学—生物化学]