高效阴离子交换色谱积分脉冲安培检测法分析注射液中的氨基酸  

Amino acids analysis in injections with high-performance anion-exchange chromatography and integrated pulsed amperometry detector

作　　者：梁立娜[1] 史亚利[1] 蔡亚岐[1] 牟世芬[1] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085

出　　处：《分析试验室》2009年第9期24-27,共4页Chinese Journal of Analysis Laboratory

基　　金：国家863重大课题(2007AA06A407);自然科学基金(20621703;20877079)项目资助

摘　　要：建立了高效阴离子交换色谱积分脉冲安培检测器同时分离并测定注射液中18种常见氨基酸、氨基己酸和牛磺酸含量的方法。注射液用20mg／L的NaN3溶液稀释1000倍，经0．22um尼龙膜过滤后直接进样分析。以一定浓度的NaOH和NaAc溶液为淋洗液，选择合适的梯度淋洗条件，20种氨基酸在AminoPacPA10阴离子交换色谱柱上在30min内很好地分离，并用脉冲安培检测器进行了测定。氨基酸的检出限在0．14～3．81pmol（25uL进样，峰面积定量）。In the present paper, twenty amino acids, including 18 common amino acids, ε-aminocaproic acid and tanfine, have been separated and detected by high-performance anion-exchange chromatography with integrated pulsed amperometric detector. Injections were diluted 1000-fold with 20 mg/L NaN3 , and then filtered with 0.22 tan nylon membrane before injection and separation with NaOH and NaAc gradient elution. Twenty amion acids were separated in 30 min on anion-exchange column and then detected by integrated pulsed amperometric detector. The detection limits range from 0.14 to 3.81 pmol （25 uL injection, calculated as peak area）. The proposed method has the advantages of simple operation, high sensitivity, good reproducibility, no interference and no need for derivatization.

关 键 词：氨基酸 高效阴离子交换色谱 积分脉冲安培检测器 注射液 

分 类 号：O657.7[理学—分析化学]