ANXA1在孕早期绒毛和蜕膜组织中的表达作用  

The Expression of ANXA1 between Deciduas and Villus in the First Trimester Pregnancy

作　　者：李红梅[1] 刘学庆[1] 余秋波[1] 董艳玲[1] 丁裕斌[1] 王应雄[1] 何俊琳[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生物学研究室,重庆400016

出　　处：《细胞生物学杂志》2009年第4期553-558,共6页Chinese Journal of Cell Biology

基　　金：重庆市自然科学基金重点项目(渝科发计字[2004]47号)资助~~

摘　　要：采用二维聚丙烯酰胺凝胶电泳(2D-PAGE)分析妊娠早期胚胎绒毛和子宫蜕膜组织细胞的蛋白质组。结果发现一个等电点约6.6、分子量约38kDa的蛋白质点在蜕膜组织中表达明显下调,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)测定其胶内酶解后的肽质量指纹谱(peptide mass fingerprint,PMF)和数据库搜索鉴定此蛋白质点为膜联蛋白-A1(ANXA1)。进一步采用RT-PCR、Western印迹和免疫组织化学技术分析了ANXA1在胚胎绒毛和子宫蜕膜组织细胞中的表达情况,证实了ANXA1 mRNA水平及其蛋白质在蜕膜组织细胞中呈现低水平表达,实验结果提示ANXA1可能在滋养细胞浸润和胎盘形成过程中发挥重要作用。Differential expression of ANXA1 in villus and deciduas from the first trimester pregnancy was investigated by two-dimensional gel electrophoresis and image analysia. The result showed the expression of a protein spot （isoelectric point is about 6.6, molecular weight is about 38 kDa） decreased significantly in deciduas. This protein spot was identified as ANXA1 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database searching. Low level expression of ANXA1 was further cornfirmed in deciduas by RT-PCR, Western blotting and immunohistochemistry. The observation suggested that ANXA1 might play an important role in regulation the invasion of trophoblast and placentation during the early pregnancy.

关 键 词：膜联蛋白-A1 绒毛 蜕膜 蛋白质组学 胚胎植入 

分 类 号：Q492[生物学—生理学]