Neuropilin-1在胰腺导管癌及MIA PaCa-Ⅱ细胞系中的表达及意义  

Expression of neuropilin-1 in human pancreatic ductal carcinoma and MIA PaCa- Ⅱ cell lines

作　　者：孟令新[1] 丁兆军[1] 陈希平[1] 费立升[1] 迟玉华[1] 赵智刚[2] 王晓芳[2] 郝希山[2] 

机构地区：[1]山东省日照市人民医院肿瘤科日照市肿瘤治疗中心,山东日照276826 [2]天津医科大芋附属肿瘤医阮

出　　处：《中国医师杂志》2009年第8期1029-1031,共3页Journal of Chinese Physician

基　　金：山东省日照市人民医院博士科研启动基金资助项目（080901）

摘　　要：目的探讨Neuropilin-1在胰腺导管癌组织及MIAPaCa-Ⅱ胰腺癌细胞系中的袁达及意义。方法运用免疫组化和RT—PCR法分别检测正常胰腺组织、癌旁组织、胰腺癌组织及MIAPaCa-Ⅱ细胞系中Neuropilin-1蛋白及mRNA表达水平。结果蛋白水平可见正常胰腺组织无表达，癌旁组织轻度表达，而胰腺癌组织及MIAPaCa-Ⅱ胰腺癌细胞中高水平表达。神经组织也可表达Neuropilin-1。mRNA水平见正常胰腺组织微量表达，癌旁组织中度表达，而胰腺癌组织及MIAPaCa-Ⅱ胰腺癌细胞中高水平表达。结论Neuropilin-1可能参与了胰腺癌的发生发展，在胰腺癌神经转移中可能起着重要作用。Objective To investigate the expression of Neuropilin-1 in human pancreatic ductal adenocarcinoma （PDAC） specimens and cell lines. Methods Expression and distribution of Neuropilin-1 were detected in operation tissue specimens of human pancreatic ductal adenocarcinoma with HE, immunohistochemistry and RT-PCR. Results Immunohistochemical analysis revealed that Neuropilin-1 were expressed in human pancreatic adenocarcinoma specimens, precancerous tissue and MIA PaCa-Ⅱ cell lines, but was absent in normal pancreatic tissue, mRNA of Neuropilin-1 could also be detected the in human pancreatic adenocarcinoma specimens, precancerous tissue and MIA PaCa- Ⅱ cell Lines, while could not be detected in normal pancreatic tissue. Neuropilin-1 expression in pancreatic adenocarcinoma were higher than that in normal pancreas, and the differences were significant（ P 〈 0. 01 ）. Conclusions Neuropilin-1 might play an potential role in carcinogenesis for pancreatic cancer. Neuropilin-1 may be relevant to perineural invasion of pancreatic carcinoma by some signal transduction.

关 键 词：神经纤毛蛋白质1/生物合成 胰腺肿瘤/代谢 

分 类 号：R735.9[医药卫生—肿瘤]