雷公藤氯内酯醇对阿尔茨海默病大鼠小胶质细胞活化的影响  

Effect of tripchlorolide on microglia activation in Alzheimer disease rat

作　　者：陈龙飞[1] 汪晓军[2] 许国英[1] 李智文[1] 

机构地区：[1]福建医科大学附属第一医院神经内科,福州350005 [2]福建医科大学病理学教研室,福州350005

出　　处：《解剖学杂志》2009年第4期485-487,501,共4页Chinese Journal of Anatomy

基　　金：福建省中医药重点课题（wzz10602）

摘　　要：目的：探讨雷公藤氯内酯醇对阿尔茨海默病大鼠学习记忆障碍及小胶质细胞活化的影响。方法：通过大鼠海马注射β类淀粉蛋白（Aβ）1-40建立阿尔茨海默病大鼠模型，Morris水迷宫测定大鼠学习记忆能力，免疫组织化学方法检测小胶质细胞。结果：Aβ组大鼠较假手术组逃避潜伏期延长，而跨越原平台位置次数明显减少；T4组大鼠较Aβ组第2天～5天平均逃避潜伏期明显缩短，跨越原平台位置次数明显增多。Aβ组大鼠海马小胶质细胞较假手术组明显增多；T4组大鼠海马小胶质细胞较Aβ组明显减少。结论：雷公藤氯内酯醇可以抑制Aβ所致小胶质细胞活化，改善阿尔茨海默病大鼠的学习记忆能力。Objective： To explore effects of tripchlorolide on learning and memory impairment and microglia activation in Alzheimer disease rat. Methods： Alzheimer disease （AD） model was established by injecting Aβ 1-40 into rat hippocampus. Morris water maze test was used for rat behavioral study, and immunohistochemistry was adopted to assess microglia in the hippocampus. Results： The mean escape latency in Aβ group was obviously increased and the frequency of passing through the platform was visibly decreased compared with the sham operated group; the mean escape latency of T4 group was obviously decreased on day 2-5 of place navigation test and the frequency of passing through the platform was visibly increased compared with the Aβ group. The number of microglia was increased significantly in the hippocampus of the Aβ group compared with the sham operated group; the number of microglia was decreased significantly in the hippocampus of the T4 group compared with that in the Aβ group. Conclusion： Tripehlorolide could inhibit microglia activation induced by Aβ 1-40 and improve learning and memory ability in AD rat.

关 键 词：雷公藤氯内酯醇 阿尔茨海默病 β-类淀粉蛋白 小胶质细胞 大鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R392.4[医药卫生—临床医学]