高压静电场对贯叶连翘种子休眠破除及药用成分的影响  

Effects of High Voltage Electrostatic Field Treatment on Seed Dormancy Breaking and Medicinal Contents of Hypericum perforatum L.

作　　者：刘晓娜[1] 张秀清[2] 李保明[1] 王愈[3] 

机构地区：[1]中国农业大学农业部设施农业工程重点开放实验室,北京100083 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《西北植物学报》2009年第8期1620-1623,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(20506029);北京市教育委员会共建项目计划(XK100190650);北京市教育委员会都市农业学科群建设项目(XK100190553);中国博士后科学基金(20080440447)

摘　　要：将高压静电场(HVEF)技术应用于贯叶连翘种子休眠破除及组培苗培养,结果显示,贯叶连翘种子在静电场(100 kv/m)中处理1 h,其发芽势(SPO)和发芽率(SPT)是对照组(无静电处理)的5.69倍和2.45倍;贯叶连翘组培苗在静电场(135 kv/m^150 kv/m)中处理1 h,其药用成分总金丝桃素含量和贯叶金丝桃素含量是对照组的1.35~1.53倍;静电场(150 kv/m)处理后,POD活性是其对照的1.30倍。研究表明,一定强度的静电场作用,能有效提高贯叶连翘种子发芽率,促进总金丝桃素和贯叶金丝桃素代谢含量。In order to break seed germination dormancy and increase medicinally metabolite production, we utilized the technology of high voltage electrostatic field （HVEF） on Hypericum perforatum L.. After dormant seeds were treated in HVEF （100 kv/m） for one hour,the seed sprouting potential （SPO） and sprouting percentage （SPT） were 5.69 and 2.45 times higher than that in the control. After micro-shoots were treated in HVEF （135 kv/m-150 kv/m） for one hour,the production of total hypericins and hyperforin was 1. 35- 1. 53 times higher than that in the control. Meanwhile, POD activity of micro-shoot in HVEF （150 kv/m） treatment was 1.30 times higher than that in the control. The results indicated that treatment of HVEF in a definite intensity for specific time was effective to stimulate seed germination and increase the production of total hypericins and hyperforin in H. perforatum.

关 键 词：高压静电场 贯叶连翘 休眠破除 金丝桃素 贯叶金丝桃素 

分 类 号：Q947.8[生物学—植物学]