铁超负荷在大鼠酒精性肝病发病中的作用  

Iron overload in the pathogenesis of alcoholic liver disease in rats

作　　者：王晓[1] 栾伟华[1] 朱玉群[1] 徐有青[1] 王晨[1] 

机构地区：[1]首都医科大学附属北京天坛医院,北京100050

出　　处：《山东医药》2009年第32期8-9,共2页Shandong Medical Journal

基　　金：北京市自然科学基金资助项目(7082031)

摘　　要：目的探讨肝组织铁超负荷在酒精性肝病(ALD)发病中的作用。方法48只健康Wistar大鼠随机分为对照组(A组)和酒精灌胃组(B组)。A组和B组根据实验时间又分为5周和10周组(A5、A10、B5、B10)。分别测定各组大鼠血清ALT、AST,取肝组织行普鲁士兰染色观察铁颗粒沉积,并观察肝脏组织学改变。结果B5和B10组大鼠血清ALT和AST分别较A5和A10组升高,且B10组高于B5组;A组大鼠肝组织未见铁颗粒沉积,B组大鼠肝组织可见铁颗粒沉积,且B10组较B5组严重;B组大鼠肝组织可见脂肪变性和炎细胞浸润,且B10组更严重。结论铁超负荷在ALD的发病中起重要作用。Objective To explore iron overload in the pathogenesis of alcoholic liver disease （ALD）. Methods Forty-eight male Wistar rats were randomized into two groups： control group （group A） and model group （group B）. Rats in group A and group B were respectively divided into 5-week group and 10-week group （ group 3.5, group AIO, group B5 and group BIO）. The serum levels of ALT and AST in each group were measured. The hepatic iron accumulation was detected by using Perls＇ prussian blue stain and pathological changes of liver tissue were examined. Results The serum levels of ALT and AST in group B5 and group B10 were both higher than those in group A5 and group A10. Moreovers, the serum levels of ALT and AST were higher in group B10 than in group B5. Excess iron accumulation was found in hepatic tissues of group B instead of in group A. Hepatic iron accumulation was much more in group B10 than that in group BS. Alcohol fatty liver and alcohol hepatitis could be observed in group B and the pathological changes were more severe in group BIO. Conclusion Iron overload may play an important role in the pathogenesis of ALD.

关 键 词：肝疾病 酒精性 铁超负荷 铁沉积 

分 类 号：R575[医药卫生—消化系统]