PEP-1-SOD对缺血再灌注损伤大鼠脑组织线粒体能量代谢及ATP酶活性的影响  

Effects of PEP-1-SOD on Mitochondrial Energy Metabolism and ATP Enzyme Activity of Brain in Rats with Ischemia Reperfusion Injury

作　　者：孙洁[1] 周艳梅[1] 王金堂[1] 李智慧[1] 

机构地区：[1]郧阳医学院附属人民医院儿科,湖北十堰442000

出　　处：《郧阳医学院学报》2009年第4期334-336,共3页Journal of Yunyang Medical College

摘　　要：目的:研究PEP-1-SOD对缺血再灌注损伤(IRI)大鼠脑组织线粒体能量代谢及ATP酶活性的影响,探讨其脑损伤的保护机制。方法:27只SD大鼠制作缺血再灌注损伤模型,随机分为缺血再灌注(IRI)组、PEP-1-SOD组和SOD组,测定大鼠脑海马组织ATP、ADP、AMP含量及Na+-K+-ATP酶和Ca2+-ATP酶活性。结果:PEP-1-SOD组ATP、ADP、AMP含量显著高于IRI组及SOD组(分别为P<0.01和P<0.05);SOD组ATP酶活性较IRI组明显升高(P<0.01),PEP-1-SOD组ATP酶活性显著高于SOD组(P<0.05)。结论:PEP-1-SOD可穿透血脑屏障,增加缺血再灌注损伤大鼠脑组织线粒体ATP含量及ATP酶活性,从而保护脑组织。Objective To investigate the effects of PEP-I-SOD on mitochondrial energy metabolism and the ATP enzyme activity of cerebral tissue in rats with ischemia reperfusion injury, and to explore the possible mechanism of cerebral protection. Methods Twenty-seven SD rats were prepared for ischemia reperfusion injury model and randomly divided into cerebral ischemia-reperfusion injury（IRI） group, PEP-I-SOD group and SOD group. The contents of ATP, ADP, AMP and the activities of Na^＋-K^＋-ATPase and Ca^2＋ -ATPase in hippocampus were determined. Results Levels of ATP, ADP, AMP in hippocampus in PEP-I-SOD group were significantly higher than those in IRI group and SOD group（ P 〈0.01 ,P 〈0.05 ,respectively） ;the ATPase activity was significantly increased in SOD group than that in IRI group （P 〈 0.01 ）, the ATPase activity in PEP-1 -SOD group was significantly higher than that in SOD group（ P 〈 0.05 ）. Conclusion PEP-1 -SOD could pass through blood brain barrier and increase the cerebral ATP content and ATPase activity,which may play an important role in the cerebral protective effect.

关 键 词：细胞穿膜多肽 缺血再灌注损伤 线粒体 ATP酶 

分 类 号：R741[医药卫生—神经病学与精神病学]