荧光染色药敏法在急性白血病中的研究  

Study on fluorescence staining assay sensitivity test in acute leukemia

作　　者：孔宪明[1,2] 缪金明[1,2] 欧阳仁荣[1,2] 肖家祁[1,2] 丁建青[1,2] 朱佑明 胡宝瑜[1,2] 童善庆 

机构地区：[1]上海第二医科大学仁济医院血液科 [2]上海第二医科大学微生物学教研室

出　　处：《上海医学》1998年第8期435-438,共4页Shanghai Medical Journal

摘　　要：目的：研究荧光染色药敏法在急性白血病中的应用。材料和方法：用新建立的荧光染色法及甲基噻唑基四唑染色（ＭＴＴ）法检测１５例初治及复发的急性白血病病例对７种常用化疗药物的体外敏感性。结果：荧光染色及ＭＴＴ法与体内疗效的阳性符合率分别为：１００％，８６％，阴性符合率分别为：６７％，６０％；特异性分别为１００％，７５％；敏感性分别为：７８％，７５％；准确率分别为８５％，６９％。结论：荧光染色法简便，快速，经济，敏感，较ＭＴＴ法具一定优越性，对指导急性白血病的个体化治疗有较高实用价值。Objective: This study was carried out to search for a better chemosensitivity assay for clinical use and also to provide guideline for individualized therapy of acute leukemia. Methods: We established and used a new apoptosis fluorescence staining assay and MTT assay in 15 cases of primary and relapsed acute leukemia and tested for their sensitivities to 7 routinelyused chemotherapeutic drugs. Results: The positive concordance rates in vivo therapy between the apoptosis fluorescence staining assay and the MTT assay were 100%, 86% respectively; the true negative rates being 67%, 60%; the specificity being 100%,75%; and the sensitivity was 78%, 75%;and the predictive accuracy rate being 85%, 69%. Conclusion: It is suggested that the apoptosis fluorescence staining assay, is sensitive, easy to perform and economically more advantageous than the MTT assay, and being of practical value.

关 键 词：急性白血症 荧光染色药敏法 药物疗法 

分 类 号：R733.710.5[医药卫生—肿瘤] R969.4[医药卫生—临床医学]