CD_3AK细胞的培养与抗肿瘤活性研究  

CULTURE OF CD_3AK CELLS AND STUDY OF THEIR ANTITUMOR ACTIVITY

作　　者：强新[1,2,3,4] 于王交 宋修歧[1,2,3,4] 丁守怡[1,2,3,4] 

机构地区：[1]青岛医学院微生物学教研室 [2]青岛市市立医院妇产科 [3]青岛医学院第二附属医院外科 [4]青岛医学院肿瘤研究室

出　　处：《青岛医学院学报》1998年第3期163-165,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省卫生厅青年科学基金

摘　　要：目的体外扩增经ＣＤ３单抗（ＣＤ３ＭｃＡｂ）活化的杀伤细胞（ＣＤ３ＡＫ），并测定其抗肿瘤细胞作用和表型变化。②方法取１３例肺癌和２例肝癌病人外周血，经密度梯度离心获取淋巴细胞，以ＣＤ３ＭｃＡｂ和ＩＬ－２作为诱导剂，采用液相三步扩增方法扩增培养，并分别用ＭＴＴ和ＩＦＡ法与自体ＬＡＫ对照细胞比较细胞动力学、细胞毒作用和表型变化。③结果ＣＤ３ＡＫ细胞扩增速度、数量和抗肿瘤活性均优于自体ＬＡＫ细胞，其表型为ＣＤ３，ＣＤ４，ＣＤ８和ＣＤ１６的特殊异源淋巴细胞群。其中ＣＤ４亚群表型数量随培养时间延长明显升高（χ２＝７．６６，Ｐ＜０．０１），而ＣＤ１６则明显下降（χ２＝５．２３，Ｐ＜０．０５），该亚群表型变化可能与培养时间有关。④结论ＣＤ３ＡＫ细胞具有较强的杀肿瘤细胞作用，同时也可作为基因治疗的理想载体细胞。Objective To amplify CD_3AK cells in vitro, and check their activity of killing tumor cells and the change of lymphocytic phenotype. Methods The peripheral blood was obtained from 13 pulmonary carcinoma and 2 liver cancer patients. Lymphocyte was separated by density gradient centrifugation, and suspended in medium with CD_3McAb and IL-2 as inducing agents. The cells were proliferated by a special method of liquid threestep amplifications. The cellular dynamic, cytotoxic activity and lymphocyte phenotype were studied by MTT and IFA methods and compared with autoLAK cells. Results The proliferation speed and the number of CD_3AK cells and their antitumor activity were significantly higher than those of autoLAK cells. The phenotypes of CD_3, CD_4, CD_8 and CD_16 were special subpopulations of heterogenous lymphocytes, among which the subtype CD4, increased significantly along with the prolongation of culture time(χ2=7.66,P<0.01), while subtype CD_16 decreased significantly(χ2=5.23,P<0.05). Conclusion CD3AK cells had strong antitumor activity and could be used as ideal carrier for gene therapy in the future.

关 键 词：细胞毒实验 CD3AK细胞 肿瘤 细胞培养 

分 类 号：R73－35[医药卫生—肿瘤]