子宫内膜癌多药耐药基因和相关蛋白基因的表达及其意义  

EXPRESSION AND IMPLICATION OF MULTIDRUG RESISTANCE GENE AND MULTIDRUG RESISTANCEASSOCIATED PROTEIN GENE IN ENDOMETRIAL CARCINOMA

作　　者：罗兵[1] 王言奎[1] 刘东光 戴淑真[1] 丁守怡[1] 张敏[1] 

机构地区：[1]青岛医学院

出　　处：《青岛医学院学报》1998年第3期169-171,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：青岛市科委资助

摘　　要：目的探讨子宫内膜癌组织中多药耐药基因（ＭＤＲ１）和多药耐药相关蛋白（ＭＲＰ）基因的表达及其意义。②方法利用半定量逆转录聚合酶链反应（ＲＴ－ＰＣＲ），对３７例初治子宫内膜癌及１０例正常子宫内膜组织ＭＤＲ１和ＭＲＰ基因的表达进行检测。③结果３７例子宫内膜癌和１０例正常子宫内膜组织均有ＭＤＲ１和ＭＲＰ基因的表达，两种基因在这两种组织中的表达水平无显著性差异（ｔ＝１．１７，１．５１，Ｐ＞０．０５）。有７例子宫内膜癌ＭＲＰ基因呈高水平表达（１．６８±０．１７），明显高于其他３０例病人（０．８３±０．１１），差异有极显著性（ｔ＝１６．５５，Ｐ＜０．００１）。④结论子宫内膜癌对化疗的耐药可能是由于固有性多药耐药所致，与ＭＤＲ１和ＭＲＰ基因的表达有关。Objective To study the expression and implication of multidrug resistance gene(MDR1) and multidrug resistance associated protein (MRP) gene in endometrial carcinoma. Methods Using semiquantitative reverse transcription PCR(RT-PCR), the expression level of both MDR1 and MRP genes were detected in endometrial carcinoma from 37 primary untreated patients (1995.7—1997.10) and 10 physiological endometriums. Results The expression of both MDR1 and MRP genes were detected in all endometrial carcinoma samples, as well as in all normal endometrium,the differences of the expression level of both MDR1 and MRP genes between the endometrial and normal endometrium were not significant(t=1.17,1.51,P>0.05).High expression of MRP gene(1.68±0.17) was shown in 7 endometrial carcinoma samples,which was much higher than that of other 30(0.83±0.11) ones (t=16.55,P<0.001). Conclusion The multidrug resistance(MDR) in endometrial carcinoma may be an intrinsic one, and it may be related to the expression of MDR1 and MRP genes.The overexpression of MRP gene may be as a prognostic factor for endometrial carcinoma patients.

关 键 词：子宫内膜癌 基因表达 多药耐 MRP 

分 类 号：R737.330.2[医药卫生—肿瘤]