电针刺激对脊髓背角组织中神经营养活性物质的影响  

The Effect of Electroacupuncture Stimulation an NeurotrophicSubstance in Cat Spinal Dorsal Horn

作　　者：张新胜 周雪 吴良芳 保天然 

出　　处：《华西医科大学学报》1998年第3期264-268,共5页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金;国家教委博士学科点专项基金

摘　　要：为进一步探讨损伤脊髓侧支出芽的机理，采用聚丙烯酰胺凝胶电泳和改良的悬滴培养法观察电针刺激对备用背根猫脊髓背角组织大于５０ｋｄ组份中神经营养活性物质的影响，以及该物质的生物活性。结果发现：①针刺实验组相对迁移率（ＲＭ）０．１蛋白质含量较对照组明显增加，ＲＭ０．５蛋白质含量明显减少。将其与本室另一非针刺资料相比，ＲＭ０．１蛋白质含量在针刺手术组较非针刺手术组明显增多，而ＲＭ０．５蛋白质含量无显著性变化，表明针刺可促进ＲＭ０．１蛋白质含量增加。②在培养中，随着ＲＭ０．１蛋含量的增多，促进鸡胚背根节神经突起生长的活性也增强，表明此蛋白质具有促进神经突起生长的活性；ＲＭ０．５蛋白质对神经突起的生长没有明显影响。For exploration of the mechanism of collateral sprouting from injured spinal cord, polyacrylamide gel electrophoresis (PAGE) and modified hanging drop culture method were used to examine the effect of acupuncture on the amount and the biological activity of neurotrophic substance in larger than 50 kd fraction of dorsal horn in cat subjected to partial dorsal rhizotomy. The results showed that the amount of protein in relative mobility (RM) 0.1 zone increased significantly in the acupunctureoperated group by PAGE and that of protein in RM 0.5 zone decreased significantly as compared with the unoperated group. While this data was compared with the data of another operated group in our lab, the amount of RM 0.1 protein of the acupunctureoperated group was significantly higher than that of the latter group; however, no obvious difference was noted between the two groups in the amount of RM 0.5 protein. It suggests that acupuncture may promote tha amount of RM 0.1 protein to increase, but acupuncture has no influence on the amount of RM 0.5 protein. Also, the results showed that, in culture, accompaning with the increase in amount, the activity of RM 0.1 protein for promoting DRG neuriteoutgrowth increased. This indicates that the RM 0.1 protein has a function to promote DRG neuriteoutgrowth, but the RM 0.5 protein has no obvious effect on DRG neuriteoutgrowth.

关 键 词：电针 背根 脊髓损伤 背角 DRG 

分 类 号：R651.2[医药卫生—外科学] R245.97[医药卫生—临床医学]