大鼠大脑中动脉暂时性闭塞后脑毛细血管内皮细胞凋亡  

Brain capillary endothelial cells apoptosis after temporary middle cerebral artery occlusion in rats

作　　者：陈兴洲[1] 陆兵勋[1] 石向群[1] 姜泊[1] 

机构地区：[1]第一军医大学南方医院神经科

出　　处：《中风与神经疾病杂志》1998年第4期195-197,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金

摘　　要：目的研究大鼠大脑中动脉（ＭＣＡ）短暂闭塞后血脑屏障（ＢＢＢ）通透性与脑毛细血管内皮细胞（ＢＣＥＣｓ）凋亡。方法大鼠ＭＣＡ经腔内导入单线闭塞不同时间后再灌流２４ｈ，血管源性脑水肿（ＶＢＥ）的程度用分光光度法测定Ｅｖａｎｓ蓝（ＥＢ）渗出量评价，凋亡细胞用原位末端标记法观察。结果ＥＢ外渗量、凋亡的ＢＣＥＣｓ数显著高于对侧，随缺血时间的延长而增加，ＥＢ外渗量与凋亡的ＢＣＥＣｓ数显著相关。Objective To examine the bloodbrain barrier (BBB) permeability and the number of apoptotic brain capillary endothelial cells (BCECs) after temporary middle cerebral artery (MCA) occlusion (MCAO) in rats.Methods MCAO was induced by cannulation with a monofilament nylon suture.The BBB permeability was assessed by quantitation of the extravasation of Evans blue dye (EB) by means of optical densitometry following formamide extraction.Results Both the extravasation of EB and the number of apoptotic BCECs increased with time of focal cerebral ischemia.The extravasation of EB in the ischemic hemisphere correlated well with the number of apoptotic BCECs in a coronal.Conclusion The apoptosis of BCECs may be involved in the mechanism of the BBB disruption.

关 键 词：脑缺血 血管源性脑水肿 血脑屏障 内皮细胞 凋亡 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R742.7[医药卫生—临床医学]