艾灸对实验性RA大鼠海马下丘脑垂体GR表达影响的研究  

One Study of the Effects of RA Rats' Glucocorticoid Receptor Expression in Hippocampus Hypothalamus and Pituitary by Moxibustion

作　　者：刘旭光[1] 周海燕[1] 黄迪君[1] 罗荣[1] 魏铮[1] 杨露晨[1] 高骏[1] 余小夏[1] 陈婷[1] 

机构地区：[1]成都中医药大学,四川成都610072

出　　处：《中华中医药学刊》2009年第9期1797-1799,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672722)

摘　　要：目的:在观察艾灸对实验性RA大鼠抗炎效应的基础上,研究艾灸对海马、下丘脑及垂体GR表达的影响,探索艾灸治疗RA的GR机制。方法:将雄性SD大鼠随机分为空白对照组、模型组、艾灸组,用福氏完全佐剂塑造大鼠实验性RA病理模型,艾灸"肾俞"、"足三里"治疗,测定大鼠右后足容积,采用免疫组化法检测海马、下丘脑、垂体GR表达。结果:造模后模型组大鼠足肿胀度明显高于空白对照组(P<0.01),艾灸组大鼠足肿胀度明显低于模型组(P<0.01);海马GR表达,模型组与空白组比较有增高趋势(P>0.05),艾灸组明显低于模型组(P<0.01);下丘脑GR表达,模型组明显高于空白对照组(P<0.01),艾灸组较模型组明显降低(P<0.01);垂体GR表达,模型组较空白对照组显著升高(P<0.01),而艾灸组与模型组比较无显著性差异(P>0.05)。结论:艾灸对实验性RA具有抗炎效应,艾灸对RA大鼠海马、下丘脑GR表达的调控可能是其实现抗炎效应的重要机制。Objective： On the basis of observing the moxibustion for experimental rheumatoid arthritis（RA） model rats′ anti-inflammatory effect,to study the effect of glucocorticoid receptor（GR） expression in hippocampus,hypothalamus and pituitary by moxibustion,to explore the mechanism of moxibustion to treat RA.Methods： Male Sprague-Dawley（SD） rats were randomly divided into control group,model group,moxibustion group,To produce RA animal models by Freund′s Complete Adjuvant（FCA）,to moxibust in ＂Shenshu（BL23）＂ and ＂ Zusanli （ST36）＂ acupoints. To detect their right feet capacity, and to detect GR expression in the hippocampus, hypothalamus, pituitary by immunohistochemistry method. Results ： Model group rats＇ feet swelling were significantly higher than control group （ P 〈 0.01 ）, moxibustion group rats＇ feet swelling were significantly lower than model group （P 〈 0.01 ）. About GR expression in hippocampus, model group had the markup tendency compared with control group（ P 〉 0.05 ）, but moxibustion group decreased significantly than model group （P 〈 0.01 ）. About GR expression in hypothalamus, model group was higher than control group （P 〈 0.01 ）, but moxibustion group decreased significantly than model group （P 〈 0.01 ）. About GR expression in pituitary, model group was higher than control group（ P 〈 0.01 ）, but there were no significant difference in moxibustion group and model group （P 〉 0.05 ）. Conclusion： Moxibustion for experimental RA model rats＇ has anti - inflammatory swelling. It＇s maybe the important mechanism that moxibustion effectively regulates and controls hippocampus, hypothalamus GR expression to achieve anti - inflammatory effect.

关 键 词：艾灸 类风湿关节炎 海马 下丘脑 垂体 糖皮质激素受体 

分 类 号：R-33[医药卫生]