针刺四神聪对失眠大鼠下丘脑内5-HT 5-HIAA含量的影响  

The Effect on the Content of 5-HT and 5-HIAA in the Insomnia Rat′ Hypothalamus Caused by Sishencong(EX-HN1) Acupuncture

作　　者：张殿全 孙忠人[2] 徐先伟[2] 

机构地区：[1]深圳市观澜人民医院,广东深圳518110 [2]黑龙江中医药大学,黑龙江哈尔滨150001

出　　处：《中华中医药学刊》2009年第9期1975-1977,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:观察针刺四神聪穴对失眠大鼠下丘脑内5-HT和5-HIAA含量的变化,揭示5-HT和5-HIAA参与针刺疗效的中枢机制。方法:选取雄性Wistar大鼠50只,随机分成5组:空白对照组(简称空白组)、模型对照组(简称模型组)、安定组、手针组、电针组,采用腹腔注射对氯苯丙胺酸(PCPA)制备失眠动物模型,通过7日针刺及药物组安定灌胃后,取大鼠下丘脑,通过荧光分光度法测定下丘脑内5-HT和5-HIAA含量。结果:PCPA腹腔注射后大鼠正常的睡眠—觉醒周期消失,且大鼠下丘脑内5-HT、5-HIAA的含量下降,与空白组比较有统计学意义(P<0.05)。模型组与空白组比较,下丘脑中5-HT和5-HIAA含量明显低于空白组,二者有显著性差异(P<0.01)。安定组与模型组相比,下丘脑中5-HT和5-HIAA含量无明显变化,二者无显著性差异(P>0.05)。手针组及电针组下丘脑中5-HT和5-HIAA含量均增加,与模型组相比,有显著性差异(P<0.01),且与安定组相比,有显著性差异(P<0.01)。电针组同手针组相比,下丘脑中5-HT和5-HIAA含量增加明显,二者有统计学意义(P<0.05)。结论:针刺四神聪穴可使PCPA化失眠大鼠下丘脑内降低的5-HT、5-HIAA含量升高,以电针作用为明显,这可能是实现其增加睡眠,提高睡眠质量的作用机制之一。Objective：To observe the content of 5-HT and 5-HIAA in the insomnia rats＇ hypothalamus caused by Sishencong（ EX-HN1 ） acupuncture,thus to reveal the central mechanism of the treatment for insomnia by acupuncture. Methods：Fifty male Wistar rats are divided into five groups at random：Blank control group （n = 10） ;Model control group （n = 10） ; Diazepam group （ n = 10） ; Hand acupuncture group （ n = 10 ） ; Electro-acupuncture group （ n = 10） ; Duplicate insomnia model of rats by means of PCPA abdominal injection. The animals in the two acupuncture groups will be treated with 7 days＇ acupuncture ,the animals in Diazepam group will be treated with seven days＇ diazepam intragastric administration, then study the results of the treatments by observing the content of 5-HT ,5-HIAA in the insomnia rats＇ Hypothalamus. Results ： After PCPA abdominal injection the normal sleep of rats disappeared, at the same time, the content of 5-HT and 5- HIAA in the hypothalamus of rats decreased with comparison to blank control group （ P 〈 0. 05 ）. Model group compared with the blank, the content of 5-HT and 5-HIAA in the hypothalamus of rats decreased obviously, has significance difference（ P 〈 0. 01 ）. There is no significant change between Diazepam group and Model control group （P 〉 0. 05 ）. Both hand acupuncture group and electro-acupuncture group can increase the content of 5-HT,5-HIAA in the insomnia rats＇ hypothalamus,with significance difference comparison to model control group（ P 〈 0. 05 ）, and have significance difference comparison to Diazepam group（ P 〈 0. 01 ）. Compared with hand acupuncture group, electro-acupuncture group has significance differenee（P 〈 0. 05 ）. Conclusion：Acupuncture can increase the content of 5-HT,5-HIAA in the insomnia rats＇ hypothalamus. This may be one of the mechanisms that acupuncture can promote sleep and enhance the quality of it.

关 键 词：针刺 四神聪穴 失眠 下丘脑 5-HT 5-HIAA 

分 类 号：R-33[医药卫生]