鼻咽癌EB病毒Rta／IgG抗体检测的诊断价值  

Evaluation of detection of Epstein-Barr virus Rta/IgG in nasopharyngeal carcinoma

作　　者：郑裕明[1] 蔡永林[1] 成积儒 李军[1] 莫永坤 高健全[1] 董智荣 钟青燕[1] 

机构地区：[1]广西梧州市红十字会医院中心实验室,梧州市肿瘤防治研究所,543002

出　　处：《中华实验和临床病毒学杂志》2009年第4期285-287,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：广西壮族自治区卫生厅计划课题（Z2008472）;梧州市科技计划项目（200801063）致谢：衷心感谢中山大学公共卫生学院柳青教授在统计学处理方面给予的帮助.

摘　　要：目的通过检测血清中的EB病毒Rta／IgG抗体，评价其在鼻咽癌诊断上的价值。方法收集211例未经治疗的鼻咽癌患者，413例对照组（包括203例相似症状的非鼻咽癌病例和210例健康体检者）的血清，用酶联免疫吸附法（ELISA）检测Rta／IgG抗体。应用受试者工作特征（ROC）曲线对结果进行分析评价。结果鼻咽癌组的Rta／IgG抗体rA值中位数明显高于对照组（P〈0．001）。Rta／IgG抗体检测诊断鼻咽癌的ROC曲线下面积为0．933，最佳截断点时敏感度为90．5％，特异度为90．1％。结论采用ELISA方法检测血清中Rta／IgG抗体可以作为检测EB病毒的一个新指标，并可作为鼻咽癌诊断的重要标志物之一。Objective This study was aimed to investigate the clinical value of Epstein-Barr virus （EBV） Rta/IgG in the diagnosis of nasopharyngeal carcinoma （NPC）. Methods Serum samples derived from 211 untreated patients with NPC, 413 subjects including 203 non-NPC ENT patients and 210 healthy volunteers as control were examined for the presence of antibodies directed against Rta/IgG by using enzyme-linked immnunosorbent assay （ELISA）. Receiver operating characteristic （ROC） curve was applied to perform methodical evaluation of this tumor marker. Results The rA value median of Rta/IgG in NPC group was significantly higher than one in control group （P 〈 0.001）. The area under ROC was 0.933. The sensitivity and specificity of this marker were 90.5 % and 90.1%, respectively, when the best cutoff value was defined. Conclusion Rta/IgG detected with ELISA method is a new target of EBV, and may be one of important marker for NPC diagnosis.

关 键 词：鼻咽肿瘤 疱疹病毒4型 人 抗体 肿瘤 

分 类 号：R739.63[医药卫生—肿瘤]