DEC205在幽门螺杆菌感染的巨噬细胞中的表达  

Expression of DEC205 in Helicobacter pylori-infected macrophages

作　　者：赵莹[1] 魏晓晴[1] 崔颖[1] 高颖[1] 

机构地区：[1]大连医科大学中心实验室辽宁省医学细胞分子生物学重点实验室,辽宁省大连市116044

出　　处：《世界华人消化杂志》2009年第20期2037-2041,共5页World Chinese Journal of Digestology

基　　金：辽宁省教育厅2009年度高等学校科研项目计划基金资助项目;No.2009A195~~

摘　　要：目的:探讨幽门螺杆菌(H pylori)感染与巨噬细胞表面的胞吞受体DEC205的关系.方法:用蛋白纯化法提取得到H pylori-HSP60.分别用H pylori、H pylori-HSP60和E.coli-LPS刺激单核细胞(NOMO1)后提取蛋白进行免疫印迹法分析.利用PMA将NOMO1细胞分化成巨噬细胞,再用H pylori或H pylori-HSP60刺激细胞后提取RNA进行实时PCR分析;细胞表面DEC205抗原的表达通过流式细胞术进行分析.结果:H pylori的刺激能明显引起DEC205蛋白的表达,而H pylori-HSP60或E.coli-LPS的刺激只引起了少许DEC205蛋白的表达;经PMA分化后的NOMO1细胞表面DEC205mRNA的表达增强,分化后的NOMO1细胞被H pylori或H pylori-HSP60刺激后也增强了DEC205mRNA的表达;流式细胞术结果表明NOMO1细胞在H pylori刺激后,细胞表面DEC205抗原的表达明显增加(88.10%vs7.84%,P<0.05),而且当NOMO1细胞被PMA分化后,经H pylori或H pylori-HSP60刺激后DEC205表达也有所增高(74.73%,51.31%vs43.77%,均P<0.05).结论:H pylori感染与巨噬细胞表面的胞吞受体DEC205有着密切的联系.AIM： To investigate the relationship between Helicobacter pylori （H pylori） infection and the expression of DEC205. METHODS： H pylori-HSP60 was prepared by a recombinant DNA procedure. After monocytic cells （NOMO1 line） were infected with H pylori or stimulated with H pylori-HSP60 or E.coli-LPS, whole cell protein was extracted and analyzed by Western blot. NOMO1 cells were then treated with PMA to induce their differentiation into macrophages, and infected with H pylori or stimulated with H pylori-HSP60. The expression of DEC205 mRNA was detected by real-time PCR. The expression of DEC205 protein on the surface of NOMO1 cells was analyzed by flow cytometry. RESULTS： The expression of DEC205 protein was markedly increased following H pylori infection, whereas the protein expression was only slightly upregulated after stimulation with H pylori-HSP60 or E.coli-LPS. After treatment of NOMO1 cells with PMA, the expression of DEC205 mRNA was enhanced. Subsequent in- fection with H pylori or stimulation with H pylori-HSP60 could induce the expression of DEC205 mRNA in differentiated NOMO1 cells. Flow cytometry analysis indicated that the expression of DEC205 protein was upregulated obviously on the surface of H pylori-infected NOMO1 cells （88.10% vs 7.84%, P 〈 0.05）. Moreover, H pylori infection and H pylori-HSP60 stimulation could also increase DEC205 expression in differentiated NOMO1 cells （74.73% and 51.31% vs 43.77%, both P 〈 0.05）.CONCLUSION： H pylori infection may be closely related with the expression of DEC205 on the surface of macrophages in gastric mucosa.

关 键 词：幽门螺杆菌 DEC205 巨噬细胞 

分 类 号：R573[医药卫生—消化系统] R378.911[医药卫生—内科学]