豚鼠巩膜组织M4受体的表达  

Expression of M4 receptor in sclera of normal guinea pigs

作　　者：高岩[1] 曾骏文[2] 仇爱华[3] 刘桂香[1] 

机构地区：[1]青岛大学医学院附属医院眼科,266003 [2]中山大学中山眼科中心,广州510060 [3]即墨市人民医院眼科,266200

出　　处：《眼科研究》2009年第9期765-768,共4页Chinese Ophthalmic Research

摘　　要：目的检测M4受体在豚鼠巩膜组织中的表达与分布。方法4周龄三色豚鼠21只,取7只动物眼球制备石蜡切片,免疫组织化学法检测后极部巩膜组织M4受体的表达与分布;其余动物分别提取眼巩膜组织mRNA和蛋白,RT-PCR和Western blot法检测M4受体mRNA和蛋白质的表达。结果免疫组织化学染色显示,豚鼠巩膜组织有M4受体表达;豚鼠巩膜组织表达M4受体mRNA,扩增产物约为221 bp,阴性对照未见扩增产物。Western blot检测显示豚鼠巩膜组织有免疫活性的M4受体表达明显,相对分子质量为38000。阴性对照组无阳性表达条带。结论豚鼠巩膜组织有M4受体蛋白表达。Objective Researches showed that muscarinic cholinoeeptor and its antagonist are closely related to the pathogenesis of myopia, but its target tissue and distribution and subtype are under clear. Present study aimed to detect the expression and distribution of M4 receptor in normal sclera of guinea pigs. Methods Twenty-one normal 4-week-old triad color guineas were selected in this study. Expression and distribution of M4 receptor protein in sclera were detected using immunohistochemistry and Western blot in 7 eyes, respectively. The expression of M4 receptor mRNA in sclera was detected by RT-PCR. Results The M4 receptor was detected in normal sclera, showing the brown granula in cytoplasm and cytomembrane. The receptor at mRNA level was detected in normal guineas sclera with the amplification product at 221 pb. No relevant product was detected in negative control. M4 receptor at protein level was detected in normal guineas sclera with signal band at 38 000,but no response signal was detected in negative control sample. Conclusion M4 receptor is expressed in normal guineas sclera.

关 键 词：豚鼠 巩膜 M4受体 

分 类 号：R778.11[医药卫生—眼科]