实验性近视眼巩膜整体与条带生物力学特性的对比研究  

Biomechanical property comparison of in vivo eyeball with in vitro scleral bands in experimental myopia

作　　者：高铁瑛[1,2] 王超英[1] 陈维毅[3] 王晓君[3] 王虹霞[1] 

机构地区：[1]白求恩国际和平医院眼科,石家庄050082 [2]邯郸市眼科医院眼科,056001 [3]太原理工大学生物力学研究所,030024

出　　处：《眼科研究》2009年第9期796-799,共4页Chinese Ophthalmic Research

摘　　要：目的豚鼠镜片诱导型近视眼分别进行在体眼球及离体后极部巩膜条带生物力学实验,对近视的发病机制进行探讨。方法2周龄豚鼠10只镜片诱导的方法单眼制备近视模型,实验前后检测双眼屈光度、眼轴长度。造模45 d后,用Instron力学材料实验仪分别进行在体眼球及后极部巩膜条带的力学实验,对巩膜材料的生物力学特性进行研究。结果45 d实验眼诱导出相对近视(-8.48±0.95)D,眼轴延长(1.90±0.14)mm,诱导前后差异有统计学意义(P<0.05)。刚度系数实验眼为(2.49±0.39)mmHg/μL,对照眼为(2.98±0.40)mmHg/μL;巩膜条带的蠕变率实验眼为(28.96±5.24)%,对照眼为(15.49±6.17)%;弹性模量实验眼为(1.55±0.25)Mpa,对照眼为(2.93±0.36)Mpa,差异均有统计学意义(P<0.05)。结论凹透镜诱导可引起豚鼠明显轴性近视;巩膜整体与条带的力学变化相一致。豚鼠实验性近视发生中巩膜力学指标发生了变化,抗变形能力变小、变形加大。Objective The etiology and pathogenesis of myopia are still under clear. Biomechanics is a way that to use biomechanical method and theory understand motion and deformation of life body quantitatively. This study was designed to evaluate the effect of two different mechanical indexes in guinea pigs with lens-induced myopia and discuss the pathogenic mechanism of myopia based on mechanical analysis. Methods Experimental myopia model was created in 10 lateral eyes of 10 two-week-old guinea pigs by stitching a - 10. 00 DS lenses on the skin of inner and outer canthus for 45 days,and the normal fellow eyes were as controls. The diopter and ocular axial length were examined with skiascope optometer and A sonography before and after operation. After 45 days, instron-5544 biomechanical machine was used to test biomechanical properties of the whole eyeball in vivo by measuring the ocular volume changes upon different pressure. The guinea pigs were sacrificed after experiment and 15 mm × 3.5 mm of posterior scleral bands were obtaind and stored in - 80 ℃ for the test of biomechanical properties, including stiffness coefficient, scleral band creep rate and elastic modulus. The use of experimental animals followed the Regulation for the Administration of Affair Cincerning Experimental Animals by State Sciencd and Technology Commission. Results The experimental myopia was successfully induced in the model eyes with the mean diopter of（ -5.85 ±0. 75）D, showing a significant difference in comparison with before induce （ ＋ 2.63 ±0.43 ） D （ t = 41.61 ,P 〈 0. 05 ）. The ocular axis was considerably enlonged after induce of lens in comparison with before induce （7.39 ±0.08 mm versus 9.29 ±0. 11 mm,t = 28.33, P 〈 0. 05 ）. The stiffness coefficient and elastic modulus were reduced in experimental eyes compared with control eyes（ t = 6.13, t = 8.89 ,P 〈 0. 05 ）. The scleral band creep rate was significantly increased in experimental eyes compared with control eyes（ t = 5.25,P 〈 0. 05）. Conclusi

关 键 词：近视 豚鼠 镜片诱导 巩膜 生物力学 

分 类 号：R778.11[医药卫生—眼科]