奥美拉唑联合超氧化物歧化酶治疗反流性食管炎的实验研究  

Experimental study on combination of omeprazole and SOD in the treatment of reflux esophagitis

作　　者：王志勇[1] 许树长[1] 孔炫[1] 

机构地区：[1]同济大学附属同济医院消化内科,上海200065

出　　处：《同济大学学报（医学版）》2009年第4期22-26,57,共6页Journal of Tongji University(Medical Science)

摘　　要：目的初步探讨奥美拉唑(omeprazole,OME)联合超氧化物歧化酶(superoxide dismutase,SOD)在治疗实验性反流性食管炎(reflux esophagitis,RE)的作用。方法清洁级雄性SD大鼠36只,随机分为6组(n=6):实验模型组、OME治疗组、SOD治疗组、联合治疗组(OME+SOD)、假手术组及空白对照组。实验模型组及各治疗组均半结扎Treitz韧带远端1 cm处肠管,胃食管交界处贲门肌纵行切开;假手术对照组(n=6)腹正中切开暴露腹腔脏器后缝合;空白对照组(n=6)未行任何处理。治疗各组分别于术前皮下注射药物处理,OME治疗剂量为30 mg/kg,SOD治疗剂量为3 mg/kg,术后24 h处死取食管标本。各组动物食管标本进行肉眼评分,组织病理评分,测定食管组织的SOD活性和丙二醛(malondialdehyde,MDA)浓度。结果各治疗组食管炎肉眼及病理评分均较实验组明显改善(P<0.05),奥美拉唑组与SOD组相比未见明显差异,而联合治疗组与另外两治疗组相比有好转趋势。在食管组织SOD活性、MDA浓度比较中,各治疗组均有显著改善(P<0.05),各治疗组间均未见明显差异,联合治疗组与另外两治疗组相比虽未见显著差异,但有所改善。结论OME联合SOD治疗实验性RE较单独应用OME或SOD治疗有好转趋势,提示氧化应激在RE发病机制中可能发挥着重要的作用。Objective To investigate the combined therapeutical effect of omeprazole （OME） and superoxide dismutase （SOD） on reflux esophagitis in rats. Methods Six-week-old healthy male Sprague- Dawley rats with body weight about 200 g each were used for the experiments. 36 rats were randomly divided into six groups with 6 rats in each group： reflux esophagitis control group, OME treatment group, SOD treatment group, OME ＋ SOD treatment group, sham operation group and blank group. Duodenogastroesophageal reflux was caused by half ligation of the duodenum 1 cm distal to the ligament of Treitz. A longitudinal cardiomyotomy was also performed in order to enhance gastric reflux to the esophagus. In the reflux esophagitis control group, no further treatment was performed in addition to the above surgical procedure. However, animals in the OME treatment group, SOD treatment group and OME ＋ SOD treatment groups were additionally treated with OME 30 mg/kg or SOD 100 mg/kg or both respectively. The rats in the sham operation group received a midline laparotomy alone, while rats in the blank group involved in no procedure. The rats were starved for 24 h before the experiments, but with free access to drinking water. All the pre-treated drugs were administered subcutaneously 30 min before surgical procedure. All rats in operation groups were starved of food for 24 h after surgery, but given free access to water. After 24 h, the rats were sacrificed and the entire esophagus was removed and visually examined for gross injury. The lower 0.5 cm part of the esophagus was used for the inspection of the injury of mucose under microscopy, while the other part was stored at -20 ℃ for SOD and MDA assays. Results All treated groups significantly attenuate the gross and histopathologic scores of acute reflux esophagitis （ P 〈 0.05 ）. No statistical significance was noted between OME and SOD groups, but the therapeutical effect on animals in the combined treated group was better. When comparison was carried out using SOD and

关 键 词：胃食管反流病 反流性食管炎 氧化应激 超氧化物歧化酶 大鼠 

分 类 号：R571[医药卫生—消化系统]