模拟IMRT对CNE-2细胞周期及Cyclin D1/Cyclin B1表达的影响  

Effects of modeling IMRT on cell cycle and expression of Cyclin D1/Cyclin B1 in CNE-2 cell lines

作　　者：王若峥[1] 王多明[1] 李品东[1] 黄莉[1] 吾甫尔[1] 

机构地区：[1]新疆医科大学附属肿瘤医院放疗一科,乌鲁木齐830011

出　　处：《上海交通大学学报（医学版）》2009年第8期914-917,930,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(3066046);新疆维吾尔自治区自然科学基金(200721115);新疆医科大学基金(2006-20)~~

摘　　要：目的探讨模拟调强放射治疗（IMRT）对人鼻咽低分化鳞癌细胞株（CNE-2）细胞周期及细胞周期素Cyclin D1、Cyclin B1转录水平的影响。方法CNE-2分为急速照射（ART）组和模拟IMRT组，两组分别给予6MVX线2、4、6和8Gy四个剂量点的照射；ART组的照射时间为1～3min，模拟IMRT组的完成时间为35min。另设空白对照组，不接受照射，其余条件相同。照射后12h，流式细胞术分析细胞周期分布，RT—PCR检测细胞周期素Cyclin D1和Cyclin B1的转录水平。结果在相同剂量点，模拟IMRT组G2期细胞比例均低于ART组，两组G2期细胞比例随照射剂量的增加而逐渐增加。模拟IMRT组与ART组在相同剂量点之间比较，Cyclin D1转录水平的差异均无统计学意义（P均〉0．05）；Cyclin B1转录水平的差异均有统计学意义（P均〈0．05），且随照射剂量的增加而下降，模拟IMRT组Cyclin B1的表达高于ART组。结论模拟IMRT照射时间延长对G2期阻滞作用下降，细胞周期素Cyclin B1的表达相对升高。Objective To detect the cell cycle and mRNA expression of Cyclin B1 and Cyclin D1 in human nasopharyngeal carcinoma cell line （CNE-2） with modeling intensity modulated radiation therapy （ IMRT）. Methods CNE-2 cell lines were divided into acute irradiation therapy（ART） group and modeling IMRT group, and both groups were irradiated at 2, 4, 6 and 8Gy dose levels by 6 MV X-ray. The time of irradiation in ART group was 1 to 3 min, and that in IMRT group was 35 min. Besides, blank control group without irradiation was established. The distribution of cell cycle was analysed by flow cytometry, and RT-PCR was employed to detect the mRNA expression of Cyclin D1 and Cyclin B1. Results Compared with ART group, the proportion of G2 phase of IMRT group was lower at each dose point, and gradually increased with the irradiation dose. There was no significant difference in the mRNA expression of Cyclin D1 at each dose point between these two groups （P 〉 0.05）. The mRNA expression of Cyclin B1 of IMRT group was significantly higher than that of ART group at each dose point（P 〈 0.05）, and the mRNA expression of both groups were down-regulated in a dose-dependent manner. Conclusion Modeling IMRT leads to relatively higher expression of Cyclin B1 and decreased radiation-induced G2 arrest.

关 键 词：人鼻咽低分化鳞癌细胞株 模拟调强放疗 细胞周期 细胞周期素B1 细胞周期素D1 

分 类 号：R739.63[医药卫生—肿瘤] R815.2[医药卫生—临床医学]