儿茶素分光光度法与微量法抗氧化活性研究  

Comparison of Spectro-photometric and Microplate Antioxidant Methods for Catechin

作　　者：王丽[1,2] 张丽[1,2] 康文艺[1,2] 

机构地区：[1]河南大学天然药物研究所,开封475004 [2]河南大学药学院,开封475004

出　　处：《天然产物研究与开发》2009年第4期664-666,共3页Natural Product Research and Development

基　　金：河南省科技厅基础前沿计划项目(0723004502);河南省教育厅基础研究计划项目(2008A360002)

摘　　要：为比较微量法和分光光度法的差异,以Trolox为参比物,PG、BHA和BHT为阳性对照品,比较其IC50和TEAC;以儿茶素的抗氧化活性为研究对象,对2种方法的相关性做Paired-samplettest。发现2种方法所得到的TEAC基本一致,4个标准品和儿茶素的抗氧化活性顺序一致,即:PG>儿茶素>BHA>Trolox>BHT;2种方法的相关系数r=0.998,显著性P=0.501。研究结果表明,分光光度法操作繁琐费时,用样量大;微量法操作简单快速,用样量小,准确性好,适合对微量天然产物抗氧化活性的评价。To compare microplate assay （MPA） with spectro-photometric method （ SPM ）, Trolox as reference substance, PG, BHA and BHT as positive control, was assayed by two methods. IC50 and TEAC of antioxidant activity were compared and studied. The paired-sample t test of antioxidant activity of catechin was assayed. The results showed that TEAC from two methods was almost approximate, and the antioxidant activity was also same in two methods ： PG 〉 catechin 〉 BHA 〉 Trolox 〉 BHT. The coefficient correlations of the paired samples was 0. 998 ,the significant value of the paired samples was 0.501. The resuhs showed that the SPM was tedious,time-consuming, much sample, and the MPA was simple, rapid, accurate,little sample for the screening antioxidant activity of natural products.

关 键 词：儿茶素 DPPH 微量法 分光光度法 

分 类 号：Q946.81[生物学—植物学] R965[医药卫生—药理学]