乙型肝炎病毒相关性肝癌患者的比较蛋白质组学研究  

Proteome Study of Differential Protein Expression in HBV-related Primary Hepatic Carcinoma

作　　者：刘池波[1] 梁勇[1,2] 潘春琴[1] 王海宝[1] 牟思华[1] 

机构地区：[1]台州学院医学院附属台州市立医院,台州318000 [2]台州学院医学院,台州318000

出　　处：《高等学校化学学报》2009年第9期1763-1766,共4页Chemical Journal of Chinese Universities

基　　金：国家"八六三"计划(批准号:2006AA02090406B)资助

摘　　要：筛选并鉴定了乙型肝炎病毒(HBV)相关性肝癌的血清差异表达蛋白.采用蛋白芯片及表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术对正常人与乙型肝炎病毒相关性肝癌患者术前血清分别进行检测,共发现了44个差异蛋白,其中21个上调,23个下调.通过高效液相色谱技术分离纯化其中表达差异最明显的蛋白,并进行质谱鉴定.通过蛋白功能结果分析表明,这些蛋白的差异表达可能与乙型肝炎病毒相关性肝癌的发生机制密切相关.To detect and identify differentially expressed proteins in the sera of patients with HBV-related primary hepatic carcinoma. The surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy （SELDI-TOF-MS） was used to screen for the differentially expressed serum proteins in patients with HBV- related primary hepatic carcinoma and healthy adults. CM10（weak cation exchange）chip found 44 differentially expressed protein peaks （ P 〈 0. 05 ） in HBV-related primary hepatic carcinoma group compared with those in the healthy adult group, 21 proteins in patients with HBV-related primary hepatic carcinoma are up-regulated, 23 proteins are down-regulated. Then the most differential protein sample was isolated and purified by high perfomance liquid chromatographic and was subjected to mass spectrometry analysis. They were listed as follows： Serum amyloid A and Serum amyloid A1. Identified in the study could be associated with the pathogenesis of HBV-related primary hepatic carcinoma.

关 键 词：乙型肝炎病毒相关性肝癌 表面增强激光解吸电离 蛋白质组 

分 类 号：O698.2[理学—化学] R735.7[医药卫生—肿瘤]