羊耳菊挥发油化学成分及其清除自由基的作用研究  

Chemical Composition of the Essential Oil from Inula cappa and Their Effects on Free Radicals Scavenging

作　　者：刘胜贵[1,2] 胡兴[1] 刘霞[1] 邓乐[2] 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《安徽农业科学》2009年第26期12536-12537,12666,共3页Journal of Anhui Agricultural Sciences

基　　金：湖南省教育厅科研项目(06C637)

摘　　要：[目的]研究羊耳菊挥发油的化学成分及其对自由基的清除作用。[方法]采用水蒸汽蒸馏法提取羊耳菊的挥发油,利用气相色谱-质谱联用法对该挥发油的化学成分进行分离鉴定,利用Fenton反应产生羟基自由基(.OH)及邻苯三酚自氧化产生超氧阴离子自由基(O.2-),以比色法测定羊耳菊挥发油对这2种自由基的清除作用。[结果]挥发油得率为0.486%(V/W),确定了28种化合物的结构和相对含量。挥发油主要含3-甲基-5-异丙基-甲基氨基甲酸酚酯(74.37%)、11,14-二十碳二烯酸乙酯(9.50%)和2,2-二甲基丙酸-2-[1,1-二甲基乙基]酚酯(7.59%)3种酯类化合物。挥发油对羟基自由基(.OH)和超氧阴离子自由基(O.2-)都有一定的清除能力,但清除羟基自由基(.OH)的能力较强。[结论]通过对羊耳菊挥发油化学成分的研究,为进一步开发利用该植物提供了科学依据。[ Objective ] The essential oils extracted from lnula cappa by steam distillation were analyzed by GC-MS. [ Method ] Essential oil from Inula cappa extracted by steam distillation, chemical compositions of essential oil were isolated and identificated by GC-MS method. Hy- droxyl radical（. OH）produced by Fenton reaction and superoxide anion radical （O^-）produced by pyrogallol autoxidation, the scavenging effects of essential oil from Inula cappa to two kinds of free radicals determined by colorlmetric method. [ Result] Extracting rate of essential oil from Inula cappa was 0.486% （ V/W）, structure of 20 kinds of compounds and their relative contents were confirmed. Essential oil mainly contented three main ester compounds of Phenol, 3-methyl-5- [ 1 -methylethyl ] -, methylearbamate （ 74.37% ）, 11 , 14 - eicosadienoic acid , methyl ester（9.50% ）, Propanoic acid,2,2-dimethyl-,2-[ 1,1-dimethylethyl] phenylester （7.59%）. Essential oil had a certain scavenging activity on hydroxyl radical （· OH）and superoxide anion radical （O2^-） , and had a better scavenging activity on hydroxyl radical （ ·OH ）. [ Conclusion ] Through the study on the chemical composition of essential oil from lnrda cappa, which provides scientific basis for the further de- velopment and utilization of this plant.

关 键 词：羊耳菊 挥发油 气相色谱-质谱法 清除自由基 

分 类 号：S567.239[农业科学—中草药栽培]