鳙鳃凝集素的稳定性及细胞凝集作用  

Stability and Cell Agglutination of Lectin from Gill of Bighead Carp(Aristichthys nobilis)

作　　者：盘赛昆[1,2] 顾小红[3] 汤坚[3] 杨超[2] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]淮海工学院海洋学院,江苏连云港222005 [3]食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《安徽农业科学》2009年第26期12589-12591,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为了探明鳙鳃凝集素在各种条件下的稳定性及其细胞凝集活性。[方法]测定经各种条件处理过的鳙鳃凝集素样品的血凝活性,评价其稳定性;通过镜检测定鳙鳃凝集素对微藻细胞及各种微生物细胞的凝集作用。[结果]琥珀酰化修饰、胰蛋白酶水解、SDS及β-巯基乙醇处理均使鳙鳃凝集素活性大大降低;在设定的处理浓度下,二甲基亚砜、NaC l及三氟乙酸对其活性没有影响;鳙鳃凝集素能专一地凝集雨生红球藻及哈维氏弧菌和鳗弧菌。[结论]鳙鳃凝集素稳定性受各种因素影响,能够选择性地凝集雨生红球藻及2株鱼类病原弧菌。[Object] To investigate the stability of lectin from gill of bighead carp under various conditions and cell agglutinating activity. [ Method ] Hemagglutination activity of lectin samples after treated under different condition was tested, and its stability was evaluated ; and ag- glutinating effect to the microalgae cells and microorganism were observed by an inverted microscope. [ Result] sueeinylation modified,trypsin hydrolysis, SDS and [^-mercaptoethanol treatment greatly reduced leetin from gill of bighead carp; dimethyl sulfoxide, NaC1, tri fluoroaeetic acid at a given treated concentration had no effect on its activity; leetin from gill of bighead carp agglutinated Haenateoccus pluvialis, vihrio harveyi and Vibrioauguil larum specifically. [ Conclusion ] The stability of lectin from gill of bighead carp had been effected by various factors, and can agglutinate Haenatcoceus pluvialis and two strain fish pathogenic vibrio selectively.

关 键 词：凝集素 稳定性 细胞凝集 血凝活性 

分 类 号：Q946[生物学—植物学] Q943