滨蒿内酯对大鼠哮喘模型蛋白激酶C-α基因表达影响的研究  

Effects of scoparone on the expression of PKC - α in asthmatic rats

作　　者：易震南[1] 黄仁清[1] 宋泽庆[1] 

机构地区：[1]广东医学院呼吸疾病研究所,湛江524001

出　　处：《中国实用医刊》2009年第17期16-19,共4页Chinese Journal of Practical Medicine

基　　金：广东省湛江市科技攻关项目2007C06007

摘　　要：目的观察滨蒿内酯对哮喘大鼠肺组织蛋白激酶C—α（proteinkinase Cα，PKC-α）mRNA及蛋白表达的影响，探讨滨蒿内酯治疗哮喘的作用机制。方法将40只雄性大鼠随机分成4组：正常对照组（N组）、哮喘组（A组）、地塞米松治疗组（D组）及滨蒿内酯治疗组（S组）。以卵清蛋白致敏和激发方法建立哮喘大鼠动物模型并给予相应治疗。测定N组及A组的肺功能，检测各组支气管肺泡灌洗液（BALF）中白细胞总数及嗜酸性粒细胞（Eos）计数；用逆转录一聚合酶链反应（RT—PCR）法检测肺组织中PKC—α mRNA，用免疫组化法检测其蛋白表达水平。结果大鼠致敏3周及诱发哮喘4周后，肺功能测定表现为呼气时间延长，呼气峰压明显增大；A组BALF中的白细胞总数及Eos计数、肺组织PKC—α mRNA及蛋白表达水平均较N组明显升高（P〈0．01）；滨蒿内酯治疗组哮喘大鼠BALF中白细胞总数和Eos计数较A组减少（P〈0．01）；PKC—dmRNA及蛋白表达水平较哮喘组下降，但仍较正常对照组升高（P均〈0．01），D组与S组之间差异无统计意义（P〉0．05）。结论滨蒿内酯可显著抑制哮喘大鼠模型肺组织的PKC—α表达，可明显抑制哮喘大鼠气道炎症的发生发展，对哮喘有较好的治疗作用。Objective To investigate the role of scoparone on the expression of protein kinase C- α （ PKC - α） in ovalbumin - challenged asthmatic rats. Exploring the possible mechanism of seoparone in the therapy of asthma. Methods Forty healthy male rats were randomly divided into 4 groups, control group（ group N）, asthmatic group（ group A）, dexamethasone group（ group D） and seoparone group（ group S）. The asthmatic model was established by ovalbumin（OVA） first intraperitoneal injection and subsequently challenged. Every time the group D and group S were intraperitoneally injected with dexamethasone or scoparone before OVA inhalation. 4 weeks later, the rats were executed. The numbers of total leukocytes and eosinophils in bronchoalveolar lavage fluid （BALF） were counted by optical microscope. RT - PCR was employed to detect the mRNA of PKC - α in the lung tissue, And their protein expression were measured by immunohistochemistry. Results After 3 weeks＇ sensitized and 4 weeks＇ challenged, the changes of pulmonary function were accompanied with prolonged expiratory time and elevated expiratory pressure, compared with group N,increase were significantly in stactics （ P 〈 0.01 ）. The numbers of total leukoeytes and eosinophils in group D or group S were much lower than those in group A（ P 〈0.01 ）. levels of PKC- α mRNA and PKC-α protein expression of group A were significantly increased compared with group N （ P 〈 0.01 ）. There was no statistical difference between group D and group S. Conclusions Scoparone can significantly inhibited the mRNA and protein expression of PKC - ot in pulmonary tissue, relieve airway in-flammation in asthmatic rats. Therefore, scoparone might be of potential value in the therapy of asthma.

关 键 词：滨蒿内酯 支气管哮喘 蛋白激酶C—α 

分 类 号：R-332[医药卫生] R28