高脂膳食对小鼠脑内铁调素基因表达影响  

Effects of high-fat diet on expression of brain hepcidin gene in mice

作　　者：边立立[1] 云少君[1] 贺晓娟[1] 魏守刚[1] 

机构地区：[1]首都医科大学公共卫生与家庭医学学院营养与食品卫生学系,北京100069

出　　处：《中国公共卫生》2009年第9期1112-1113,共2页Chinese Journal of Public Health

基　　金：北京市自然科学基金(7052008)

摘　　要：目的探讨高脂膳食对小鼠脑内铁稳态的影响,为通过合理膳食预防和延缓脑老化提供科学依据。方法美国癌症研究所(ICR)雄性小鼠40只随机分为4组,通过Morris水迷宫实验检测各组小鼠的空间学习记忆能力,用逆转录聚合酶链反应法(RT-PCR)检测小鼠脑内铁调素(Hepcidin,Hep)mRNA的表达水平。结果高脂膳食组、脑老化模型组、高脂膳食+脑老化模型组的Hep基因与内参基因(β-actin)电泳图灰度比值分别为(0.71±0.06),(0.75±0.03)和(0.73±0.08),均低于普通膳食对照组的(0.94±0.06),差异有统计学意义(P<0.05);而高脂膳食组、脑老化模型组和高脂膳食+脑老化模型组之间Hep mRNA表达水平无明显差异。结论高脂膳食可以下调小鼠脑内Hep mRNA的表达,与脑老化模型组类似,提示高脂膳食可能引起大脑铁稳态失衡进而导致神经退行性病变。Objective To investigate the effects of high-fat diet on iron homeostasis of brain in mice. Methods Forty adult male ICR mice were randomly divided into four groups ： high-fat diet group, high-fat diet ＋ D-galactose brain aging model group, D-galactose brain aging model group and normal control group. Learning and memory ability of the mice were detected by Morris water maze test. The hepcidin（Hep） mRNA level was detected by reverse transcription polymerase chain reaction. Results The Hep gene expression of high-fat diet group （0. 71 ± 0. 06）, high-fat diet ＋ D-galactose brain aging model group（0. 73 ±0. 08 ） and D-galactose brain aging model group （0.75 ±0. 03 ）decreased significantly compared with that of the normal control group （0.94 ±0.06, P 〈 0.05 ）. But there was no significant difference among high-fat diet group, high-fat diet ＋ D-galactose brain aging model group, and D-galactose brain aging model （ P 〉 0.05 ）. Conclusion High-fat diet can down-regulate the Hep gene expression in mice brain. High-fat diet may induce imbalance of iron homeostasis leading to neurodegenerative diseases.

关 键 词：高脂膳食 脑老化 铁调素(Hep) 

分 类 号：R332[医药卫生—人体生理学]