肿瘤特异基因跟踪检测小儿微量残留白血病的研究  

Longitudinal Detection of Minimal Residual Disease of Childhood Leukemia with Tumor Gene-specific Markers

作　　者：顾龙君[1] 马志贵[1] 薛惠良[1] 况少青[2] 董硕[2] 陈竺[2] 叶裕春[3] 蒋慧[4] 

机构地区：[1]上海第二医科大学附属新华医院儿内科,200092 [2]上海血液学研究所,200025 [3]上海市儿科研究所,200092 [4]上海市儿童医院,200040

出　　处：《上海第二医科大学学报》1998年第5期253-256,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：本课题受国家自然科学基金资助(39170364);卫生部科研基金资助(91304245)

摘　　要：应用 PCR 技术,以 SIL-TAL-1、HRX 基因相合的,和 bcr/ab1以及 PML-RAR_α4种融合基因作为肿瘤特异标志,对7例小儿急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)和16例小儿急性早幼粒细胞白血病(acute promyecytic leukemia,APL)在完全缓解(complete remission,CR)后的微量残留病(minimal residual disease,MRD)作系统的跟踪检测。结果所有的小儿 ALL 和 APL 在 CR 后均存在MRD,MRD 持续检测阴性,提示可能临床治愈,若 MRD 持续阳性或由阴转阳则提示将发生骨髓复发。检测的灵敏度是10^(-4)～10^(-6)。白血病 MRD 的跟踪检测有重要的临床意义。By using polymerase chain reaction analysis,minimal residual disease (MRD) was vertically and systematically detected in 7 children with acute lymphoblastic leukemia (ALL) with SIL-TAL-1,HRX-AFA and BCR/ABL fusion genes,and in 16 children with PML-RARo fusion gene for tumor specific markers.The results showed that all of the cases had MRD after complete remission.It may be clinically cured when the detection of MRD continuously showed negative.It would develop bone marrow relapse when MRD persisted or reappeared.The detection sensitivities of MRD were 10^(-4)～10^(-6).It is important to detect MRD in clinic.

关 键 词：白血病 ALL 微量残留病 PCR 肿瘤特异基因 

分 类 号：R733.710.4[医药卫生—肿瘤]