大蒜油诱导人胃癌细胞分化和凋亡的机制研究  

Garlic oil induces differentiation and apoptosis of human gastric cancer cell line

作　　者：李晓光[1,2,3] 谢锦玉[1,2,3] 李文梅[1,2,3] 赵敏[1,2,3] 崔建涛[1,2,3] 吕有勇[1,2,3] 

机构地区：[1]中国康复研究中心细胞分子生物学研究室 [2]中国中医研究院基础理论研究所 [3]北京医科大学肿瘤学院分子生物学研究室

出　　处：《中华肿瘤杂志》1998年第5期325-327,共3页Chinese Journal of Oncology

基　　金：国家863计划基金

摘　　要：目的从细胞和基因水平阐明大蒜油对人胃癌ＢＧＣ８２３细胞的作用机理。方法用激光扫描共聚焦显微镜及Ｎｏｒｔｈｅｒｎ杂交等技术进行分析。结果大蒜油对癌细胞形态、增殖和致瘤性有明显影响。结论大蒜油通过促进ｐ５３、ｐ２１等的表达，诱导肿瘤细胞分化，同时诱导肿瘤细胞凋亡。Objectives To study the mechanism of garlic oil induced differentiation of human gastric cancer cell line BGC 823. Methods Confocal microscopic assay and Northern blot hybridization were used to detect morphologioal change and gene expression in garlic oil treated human gastric cancer cells (BGC823). Results Cell growth in vitro and tumorigenicity in nude mice were inhibited dramatically by garlic oil. Garlic oil treatment resulted in cell apoptosis as shown by DNA fragmentation. High expression level of WAF1/p21 and wild type p53 gene was detected in garlic oil treated cells. Conclusion These data indicate that differentiation and apoptosis of BGC 823 cell can be induced with garlic oil, and p21 and p53 genes possibly play important role in the process.

关 键 词：大蒜油 胃肿瘤 细胞分化 细胞凋亡 

分 类 号：R735.201[医药卫生—肿瘤] R730.1[医药卫生—临床医学]